Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-405-2 | CAS number: 106-51-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- For the determination of the growth curve not the cell amount was used, but the fluorescence. In a pre-test the correlation of cell amount and fluorescence was confirmed.
- GLP compliance:
- no
- Remarks:
- accredited by DAkkS
Test material
- Reference substance name:
- p-benzoquinone
- EC Number:
- 203-405-2
- EC Name:
- p-benzoquinone
- Cas Number:
- 106-51-4
- Molecular formula:
- C6H4O2
- IUPAC Name:
- 1,4-benzoquinone
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- A stock solution was prepared with the test item. Different dilutions with ultrapure water were prepared thereof.
50 mL-test preparations contained 40 ml of the related dilution as well as 5 ml algal medium and 5 ml algal culture (consisting of algal medium and Desmodesmus subspicatus CHODAT). The tests were performed in duplicate over a 72 h period of time.
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 20 – 22 °C
- pH:
- 8.1 - 9.1
- Nominal and measured concentrations:
- 0,5 mg/l, 1,0 mg/l, 2,0 mg/l, 3,0 mg/l, 10 mg/l
- Details on test conditions:
- In pre-tests it was found out that a direct correlation of growth rate and measurable fluorescence exists. Hence, the measurement of the fluorescence of all samples was performed using a fluorescence measuring device (Kontron) after 0, 24, 48 and 72 h.
- Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
Results and discussion
Effect concentrations
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: fluorescence
Any other information on results incl. tables
Table 2: Fluorescence of all test samples with p-benzoquinone und control (=0 mg/l)
concentration [mg/l] |
fluorescence |
|||||||||||
0 h |
24 h |
48 h |
72 h |
|||||||||
I |
II |
mean |
I |
II |
mean |
I |
II |
mean |
I |
II |
mean |
|
0 |
2,6 |
2,5 |
2,55 |
3,4 |
3,2 |
3,3 |
6,0 |
5,1 |
5,55 |
9,6 |
9,3 |
9,45 |
0,5 |
2,5 |
2,8 |
2,65 |
2,0 |
1,8 |
1,9 |
5,1 |
4,6 |
4,90 |
6,7 |
7,0 |
6,90 |
1 |
2,8 |
3,0 |
2,90 |
3,1 |
3,2 |
3,2 |
3,9 |
4,3 |
4,10 |
6,1 |
7,3 |
6,70 |
2 |
2,8 |
2,7 |
2,75 |
2,5 |
2,9 |
2,7 |
3,3 |
3,8 |
3,60 |
5,6 |
5,5 |
5,60 |
3 |
2,4 |
2,6 |
2,50 |
2,4 |
2,6 |
2,5 |
3,0 |
2,9 |
3,00 |
4,1 |
4,2 |
4,20 |
10 |
2,0 |
2,5 |
2,25 |
1,7 |
1,7 |
1,7 |
1,2 |
1,3 |
1,30 |
1,4 |
1,4 |
1,40 |
Table 3: growth rate of Desmodesmus subspicatus CHODAT at different concentrations of p-benzoquinone in relation to control (= 100%)
concentration [mg/l] |
growth rate in 72 h of Desmodesmus subspicatus in % |
0,5 |
80,48 |
1 |
57,77 |
2 |
46,37 |
3 |
26,85 |
10 |
0,00 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The toxicity of p-benzoquinone to algae was investigated in a study acc. to OECD Guideline 201 (Alga, Growth Inhibition Test). The 72 h EC50 was determined to be 1,5 mg/L (based on recalculated initial concentrations).
- Executive summary:
The toxicity of p-benzoquinone to algae was investigated in a study acc. to OECD Guideline 201 (Alga, Growth Inhibition Test).
For the hazard and risk assessment the effect concentration for growth rate should be used: EC50 (72 h) = 1,5 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
