A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 July - 04 Aug, 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: done under OECD method and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol

EC Number:

414-810-0

EC Name:

A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol

Cas Number:

144163-85-9

Molecular formula:

Empirical formula: C25H35N2O5

IUPAC Name:

butanedioic acid; propan-2-ol; bis(tert-butyl N-[(4R)-5-amino-4-hydroxy-1,6-diphenylhexan-2-yl]carbamate)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

A sample of activated sludge plant was kept under aerobic conditions. To prepare the inoculum, the sample was allowed to settle for 30 mins and filtered through Whatman GFA course filter paper. The first 250ml was discarded.

Duration of test (contact time):

28 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Test solns were incubated in the dark at 21±1°C and analysed for dissolved oxygen at 0, 4, 7, 11, 14, 18, 21, 25 and 28 days using a Yellow Springs BOD meteer (model 59).

Reference substance

Reference substance:

other: sodium benzoate

Results and discussion

Preliminary study:

A-88820.605 attained 0% degredation after 28 days and cannot, therefore, be termed as readily biodegadeable.
There was no evidence of inhibitory effects on the bacteria inoculum at 2mg/L.

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Details on results:

Cultures containing both test and standard substances combined showed an oxygen depletion value 20% higher than that anticipated on the basis of results from separate culture on day 14. Consequently A-88820.605 is not considered to have had an inhibitory effect on sewage bacteria under the conditions of this test.

BOD5 / COD results

Results with reference substance:

Sodium benzoate attained 72% biodegredation after 28 days.

Any other information on results incl. tables

Oxygen depletions in the inoculated control series were ≤1.5 mg oxygen per litre after 28 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

A-88820.605 may not be termed as readily biodegadable

Executive summary:

A-88820.605 can not be considered readily biodegadeable.

There was no evidence of inhibitory effects under the conditions of this test.