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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
27 Mar - 30 Mar, 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Done under GLP and OECD methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
Version / remarks:
Method C3, Directive 92/69/EEC
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol
EC Number:
414-810-0
EC Name:
A mixture of: bis(1S,2S,4S)-(1-benzyl-4-tert-butoxycarboxamido-2-hydroxy-5-phenyl)pentylammonium succinate; isopropyl alcohol
Cas Number:
144163-85-9
Molecular formula:
Empirical formula: C25H35N2O5
IUPAC Name:
butanedioic acid; propan-2-ol; bis(tert-butyl N-[(4R)-5-amino-4-hydroxy-1,6-diphenylhexan-2-yl]carbamate)

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
Nominal test concentrations: 0.10, 0.22, 0.46, 1.0, 2.2 and 4.6 mg/L
Mean measured test concentrations: 0.082, 0.17, 0.41, 0.70, 2.3 and 4.5 mg/L
Each test solution was prepared in triplicate. One untreated control was also used.

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Centre of Algae and Protozoa, c/o Freshwater Biological Association, Cumbria, England.

Study design

Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
23 ± 1°C
Nominal and measured concentrations:
Nominal test concentrations: 0.10, 0.22, 0.46, 1.0, 2.2 and 4.6 mg/L
Mean measured test concentrations: 0.082, 0.17, 0.41, 0.70, 2.3 and 4.5 mg/L
Each test solution was prepared in triplicate. One untreated control was also used.
Details on test conditions:
Algae were cultured for 72hrs under continuous illumination of approx. 7,000 lux in an orbital shaker at 120 cycles /minute.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
other: EbC50 (based on comparison of area under growth curves).
Effect conc.:
0.37 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95%CL: 0.27 - 0.51 mg/L
Duration:
72 h
Dose descriptor:
other: ErC50 (comparison of growth rates)
Effect conc.:
0.72 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95%CL: 0.58 - 0.88 mg/L
Duration:
72 h
Dose descriptor:
NOELR
Effect conc.:
0.17 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
EbC50 (72 hr): 0.37 mg/L
ErC50 (0 - 72 hr): 0.72 mg/L
NOEL: 0.17 mg/L
Results with reference substance (positive control):
Mean cell density of control @ 0 hrs: 1.29 x 10 exp 4
Mean cell density of control @ 72 hrs: 7.43 x 10 exp 5

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EbC50 (72 hr): 0.37 mg/L
ErC50 (0 - 72 hr): 0.72 mg/L
NOEL: 0.17 mg/L