Morpholine, 4-[(triethoxysilyl)methyl]-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007-05-29 to 2007-07-26

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

87/302/EWG, C.11

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

480-370-1

EC Name:

-

Cas Number:

21743-27-1

Molecular formula:

Hill formula: C11H25NO4Si CAS formula: C11H25NO4Si

IUPAC Name:

4-[(triethoxysilyl)methyl]morpholine

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test item hydrolyses spontaneously in water. To reach a homogenous hydrolysis, the test item was mixed with test water and this mixture was stirred for about 24 hours at test temperature (21°C) in diffuse light before adding the inoculum. The test flask were tightly closed to avoid water loss by evaporation.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

-Origin: domestic waste water treatment plant Darmstadt, Germany
-Cultivation: The activated sludge was washed by centrifugation and the supernatant liquid phase was decantded. The solid material was resuspended in tap water and again centrifuged. This procedure was done three times. The washed activated sludge was resuspended in tap water and sewage feed. The sludge was kept at room temperature for three days. The activated sludge was aerated and fed each day during acclimatisation. Immediately before use the dry weight of the activated sludge was determined, and diluted to 3 g/L with tap water. The pH of the activated sludge was 7.8 and therefore no adjustment was necessary.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

no

Test conditions

Hardness:

not given in the report

Test temperature:

19-21 °C

pH:

7.3-8.5

Dissolved oxygen:

8.0-9.3

Salinity:

not given in the report

Nominal and measured concentrations:

nominal: 10, 32, 100, 320, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass flask with 1 litre volume and Karlsruher flasks of 250 mL volume
- Aeration: 0.8 litre per minute with compressed air

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

Up to the highest nominal test concentration of 1000 mg/l less than 20 % inhibition was noted after 3 h incubation

Results with reference substance (positive control):

The 3-hour EC50 of 3,5-Dichlorophenol was calculated to be 14.4 mg/L (based on the concentrations 3.2, 10 and 32 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

N-(Morphlinomethyl)triethoxysilane was not toxic to bacteria from activatied sludge under the reported conditions.

Executive summary:

The influence of (N-Morpolinomethyl)triethoxysilane on the activity of activated sludge was evaluated by measuring the respiratory rate under defined conditions (OECD Guideline 209) . The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of various concentrations of the test item after an incubation period of 3 hours. Based on measured inibition rates, the 3 -hour EC20 and EC50 could not be quantified because up to the highest nominal test concentration of 1000 mg/L less than 20% inhibition was noted after three hours incubation. Nevertheless the 3 -hour EC20 and EC50 are clearly higher than 1000 mg/L under the reported conditions.The positive and the inocculum control worked as expected.