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EC number: 217-691-1 | CAS number: 1931-62-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-05-07 to 2017-11-30; Experimental phase: 07 - 15 June 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- OECD 202 (13 April 2004)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: M-150801 (according to CoA from 14 March 2017)
- Expiration date of the lot/batch: 2018-04-07
- Purity test date: 13.03.2017
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Keep only in the original container. Protect from heat and direct sunlight. Store at max. +30°C
- Stability under test conditions: Not stable - Analytical monitoring:
- yes
- Details on sampling:
- Substance specific analysis was performed at the test site of biochemA GmbH.
- Nominal concentrations: 0.0 (Sample code: NC - negative control); 38.1 (Sample code: A); 61 (Sample code: B); 97.7 (Sample code: C); 156.3 (Sample code: D) and 250.0 mg/L (Sample code: E)
- Sampling method: The samples were filled into 2 mL microcentrifuge tubes and stored in the freezer (s -18 °C). The sampling and documentation is conducted according to the following specification:
* At the start of the test (0 h), the test solutions NC, A, B, C, D and E were sampled before division to the test vessels (each concentration: 4 samples of 1.5 mL).
* After 24 h exposure: Before renewal test solution, all replicates of NC, A, B, C, D and E were sampled (one sample per replicate: 4 samples of 1.5 mL per concentration).
* At the end of the test (48 h): All replicates from NC, A, B, C, D and E were sampled (one sample per replicate: 4 samples of 1.5 mL per concentration).
Of each sampled treatment, one of the samples from 0 h, 24 h (old and fresh) and 48 h is sent to the analytical laboratory at the test site. The remaining samples were stored as retain samples at s -18 °C until finalization of the study. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solutions (one for the start and one for the renewal of the test solutions at 24 h) were prepared by adding 127.4 mg (0 h) and 125.4 mg/L (24 h) test item to 500 mL test medium and stirred for 1 h on a magnetic stirrer at room temperature.
- Controls: Yes - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test organism Daphnia magna Straus originates from the clone breeding of the Federal Environment Agency, department IV 2.4, Berlin, and has been cultivated at Hydrotox GmbH since October 2012. The daphnids are cultured at 20 ± 2 °C with 16 h light: 8 h dark and transferred into fresh M4 medium twice a week. They are kept at approx. 10 animals / 200 mL and fed on workdays with Desmodesmus subspicatus algae corresponding to 0.1 mg C × daphnid -1 × day -1. The test organisms used were 3.15 – 22 h old at the start of the test.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- The temperature during the exposure was 20.6 – 21.3 °C (required 18 – 22 °C, constant within ± 1 °C)
- pH:
- The pH was 7.7 in the control and 7.4 – 7.7 in the test item treatments
- Dissolved oxygen:
- The dissolved oxygen concentration was 7.9 – 8.1 mg/L in the control and 7.6 – 8.2 mg/L in the test item treatment
- Conductivity:
- 687 µS/cm
- Nominal and measured concentrations:
- - Nominal concentrations: 0.0; 38.1; 61.0; 97.7; 156.3 and 250.0 mg/L
- Measured concentrations: 0.0; 33.2; 55.2; 92.9;153.8; 295.9 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Material, size, fill volume: 50 mL
- Aeration: Yes: After addition of the stock solutions, except the vitamin stock solution S23, the medium (before renewal / after renewal) was aerated for 2.0 h to achieve oxygen saturation.
- Type of flow-through (e.g. peristaltic or proportional diluter): No: Semi-static system.
- Renewal rate of test solution (frequency/flow rate): Yes, after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM
The test was performed with M4 medium according to OECD 202 (2004).
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light : 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility, Mortality
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
A preliminary test without GLP was performed before start of this GLP-study. Nominal concentrations of 1, 10, 50 and 100 mg/L test item resulted in 0 % immobility after 48 h exposure.
ln the present study, nominal concentrations of 38.1 — 250 mg/L test item were tested. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 191.16 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 73.06 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 92.95 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 55.24 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: not reported
- Mortality of control: no
See also table 1 " Immobility after 24 h and 48 h exposure" in "Any other information on results" incl. tables" below. - Results with reference substance (positive control):
- - Results with reference substance valid?: Yes
- Reported statistics and error estimates:
- The effect concentrations after 24 h and 48 h exposure were calculated with the statistical software ToxRat Professional 3.2.1.
- Validity criteria fulfilled:
- yes
- Conclusions:
- For the conducted semi-static acute immobilization test with Daphnia magna, applying nominal concentrations of 38.1 — 250 mg OO-tert-Butylmonoperoxymaleat/L, the following effect levels were calculated:
- 48 hr EC50 (measured): 191.16 mg/L (95%-CL: 150.00 - 271.07);
- 48 hr EC20 (measured): 101.64 mg/L (95%-CL: 71.83 - 129.06);
- 48 hr EC10 (measured): 73.06 mg/L (95%-CL: 44.38 - 96.27);
- 48 hr LOEC: 92.95 mg/L;
- 48 hr NOEC: 55.24 mg/L.
Immobilisation of the test organisms in the control at the end of the test was 0 % and therefore ≤ 10 %.
Dissolved oxygen concentration in the control and the test item treatments at the end of the test was ≥ 7.6 mg/L and therefore ≥ 3 mg/L.
The test is valid according to OECD Test Guideline 202 (13 April 2004) - Executive summary:
The 48–hr-acute toxicity of the test item to Daphnia magna Straus was studied under semi-static conditions according to OECD 202 (OECD 2003). Daphnids were exposed for 48 h to a control containing no test substance, and OO-tert-butyl monoperoxymaleate at nominal concentrations of 38.1, 61.0, 97.7, 156.3 and 250.0 mg/L and measured concentrations of 33.2; 55.2; 92.9;153.8; 295.90 mg/L (geometric mean).
Mortality/immobilization and sublethal effects were observed daily.
The 48 – hr EC50 was 191.16 mg/L.
The 48 – hr NOEC based on immobilization was 55.24 mg/L.
Based on the results of this study, OO-tert-butyl monoperoxymaleate would not be classified as toxic to Daphnia magna Straus in accordance with the CLP classification system.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age (e.g. 1st instar): 3.15 - 22 h
Test Type: Static Renewal
- 48 hr EC50 (measured): 191.16 mg/L (95%-CL: 150.00 - 271.07);
- 48 hr EC10 (measured): 73.06 mg/L (95%-CL: 44.38 - 96.27);
- 48 hr LOEC: 92.95 mg/L;
- 48 hr NOEC: 55.24 mg/L.
Reference
Table 1: lmmobility after 24 h and 48 h exposure |
|||||||||||
Nominal test item concentration [mg/L] | Number of daphnids per concentration (4 replicates a 5) |
lmmobile daphnids (replicates 1 - 4) | Immobility [%] | ||||||||
24 h | 48 h | 24 h | 48 h | ||||||||
1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | ||||
NC | 20 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
38.1 | 20 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
61.0 | 20 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
97.7 | 20 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 25 |
156.3 | 20 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 4 | 0 | 45 |
250.0 | 20 | 0 | 0 | 0 | 0 | 2 | 3 | 5 | 3 | 0 | 65 |
The initial measured test item concentrations in the test item treatments were 109 – 148 % (0 h) and 115 – 152 % (24 h new) of the nominal concentrations. Until the renewal of the test solutions and the end of the exposure period, the measured test item concentrations decreased to 67 – 92 % (24 h old) and 68 – 94 % (48 h) of the nominal concentrations.
Description of key information
For the conducted semi-static acute immobilization test with Daphnia magna Straus according to OECD 202 (OECD 2004), applying nominal concentrations of 38.1 — 250 mg OO-tert-butyl monoperoxymaleate/L, the following effect levels were calculated:
- 48 hr EC50 (measured): 191.16 mg/L (95%-CL: 150.00 - 271.07);
- 48 hr EC10 (measured): 73.06 mg/L (95%-CI: 44.38 - 96.27);
- 48 hr LOEC: 92.95 mg/L;
- 48 hr NOEC: 55.24 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 191.16 mg/L
Additional information
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