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activated sludge respiration inhibition testing
Type of information:
other: read across from analogue substance
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Test item without emulsifier was investigated.

Amidoamine (UVCB):
- Name of test material (as cited in study report): Amidoamine (UVCB)
- Substance type: Amidoamine
- Chemical name: Glycerides, C14-20, reaction products with diethylenetriamine (preliminary naming)/Amides, from diethylenetriamine and hydrogenated palm oil
- CAS 85409-11-6/1618093-67-6
- Physical state: pale yellowish solid at 20 °C
- Batch No.: K8 4309 L481
- Expiry date of batch: 09 March 2018
- Purity: 100 % (UVCB)
- Storage condition of test material: Room temperature, protected from light
- Stability: stable under test conditions
Analytical monitoring:
Test organisms (species):
activated sludge of a predominantly domestic sewage
Test type:
Water media type:
Limit test:
Total exposure duration:
3 h
Test temperature:
18 - 22 °C
The pH of the test solution was measured at the start and the end of the 3 hour incubation period. pH was betwenn 7.0 - 8.0.
Reference substance (positive control):
Key result
3 h
Dose descriptor:
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Results with reference substance (positive control):
The EC50 (3 h) of the reference item 3,5-dichlorphenol was in the range of 2 to 25 mg/l for total respiration.
Validity criteria fulfilled:
The effect of the test item on the respiration rates of STP organisms has been investigated and gave a NOEC value of 1000 mg/L, i.e. the substance is not toxic to STP microorganisms.
Executive summary:

In a valid GLP study according to OECD 209 the test item was aerobically exposed to standard OECD mineral medium and activated sludge (3 g/l) obtained from a STP (non-adapted, domestic). Exposure was for 3 hours under stirring conditions between 18 and 22°C. Glass reactors were constantly aerated with CO2 free air. Respiration rates were measured by pouring a well-mixed sample of each test vessel into a 250 mL darkened glass BOD flask after 3 hours incubation time without further aeration. The dissolved oxygen concentration was measured with an oxygen probe attached to an oxygen meter. The substance is not toxic to STP microorganisms, since NOEC for total respiratory inhibition was 1000 mg/l.

Description of key information

IC50, 3h > 1000 mg/l

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L
EC10 or NOEC for microorganisms:
1 000 mg/L

Additional information

Analogue substance was tested for toxicity to aquatic organisms following OECd 209. Under the experimental conditons IC50, 3h > 1000 mg/l.

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