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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Assessment of the safety of dioctyl adipate in freshwater environments
Author:
Felder, et al.
Year:
1986
Bibliographic source:
Environ Toxicol Chem 5:777-784

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: ASTM E-35.32 (1979) (Proposed Draft)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Bis(2-ethylhexyl) adipate
EC Number:
203-090-1
EC Name:
Bis(2-ethylhexyl) adipate
Cas Number:
103-23-1
Molecular formula:
C22H42O4
IUPAC Name:
bis(2-ethylhexyl) adipate
Test material form:
liquid
Specific details on test material used for the study:
The test substance is listed as Dioctyl adipate or di(2-ethylhexyl) adipate.
Radiolabelling:
yes

Sampling and analysis

Details on sampling:
The test concentration was confirmed by radioanalysis before the fish were introduced.

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Lepomis macrochirus
Details on test organisms:
The test organism is listed as the bluegill sunfish (Lepomis macrochirus).

Study design

Route of exposure:
aqueous
Test type:
not specified
Water / sediment media type:
natural water
Remarks:
well water
Total exposure / uptake duration:
28 d
Total depuration duration:
14 d

Test conditions

Hardness:
Hardness of 265 ± 10 mg/L,
pH:
7.8 to 8.3
Dissolved oxygen:
9.2 to 10.2 mg/L dissolved oxygen concentration
Conductivity:
700 p.0 /cm.
Nominal and measured concentrations:
The bioconcentration study evaluated the effects of the test substance with a concentration of 0.20 mg/L. The test concentration was confirmed by radioanalysis before the fish were introduced.
Reference substance (positive control):
not specified

Results and discussion

Bioaccumulation factor
Key result
Conc. / dose:
ca. 0.2 mg/L
pH:
8.05
Type:
BCF
Value:
27 dimensionless
Basis:
other: Based on 14C determinations and not on measurement of actual test substance concentrations in fish tissue
Remarks:
.
Time of plateau:
1 d
Depuration
Elimination:
yes
Parameter:
DT50
Depuration time (DT):
1 d

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The whole-fish BCF for the test substance was determined to be 27 for bluegills exposed to the concentration tested (nominal concentration of 0.20 mg/L) and 250 ± 80 ug/L [14C]DOA at test termination on day 28. This relatively low BCF indicates that fish exposed to concentrations of the substance in water or food chain will not be adversely affected.