Methacrylic anhydride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Method in accordance with OECD Guideline, GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methacrylic acid

EC Number:

201-204-4

EC Name:

Methacrylic acid

Cas Number:

79-41-4

Molecular formula:

C4H6O2

IUPAC Name:

2-methylprop-2-enoic acid

Details on test material:

purity: > 98.5 %
Batch TD 90-133

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

other: activated sewage sludge bacteria

Details on inoculum:

Aeration stage of the HRC Limited sewage treatment plant treating predominantly domestic sewage

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

3 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

other: Percent biodegradation determined by comparing oxygen depletion value with the corresponding ThOD

Details on study design:

Sampling at 0, 5, 15 and 28 days
20°C

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

86

Sampling time:

28 d

Remarks on result:

other: Oxygen depletion = 4.325 mg O2/L; ThOD = 5.01 mg O2/L

Details on results:

Oxygen depletion in the inoculated and non-inoculated control series were within prescribed limits.
The substance passed the "time window" criterion (60% degradation within 10 days of attaining the 10% level)

BOD5 / COD results

Results with reference substance:

87% degradation within 28 days

Any other information on results incl. tables

Mean oxygen depletion and percentage biodegradation values

Culture medium	Day 5	Day 15	Day 28
(a) Dilution water without inoculum     02depletion (mgO2/I)	0.175	0.275	0.250
(b) Dilution water with inoculum     02depletion (mgO2/I)	0.100	0.300	0.375
(c) Test substance (3 mg/l)     02depletion (mgO2/I)     % degradation	3.050 61	3.000 60	4.325 86
(d) . Standard substance (3 mg/l)     02depletion (mgO2/I)     % degradation	3.275 65	3.450 69	4.375 87

t1/2: 10.3 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

In a valid guideline study, the substance was considered readily biodegradable (86% degradation within 28 days, 10 day window passed).

Executive summary:

In a valid guideline study acc. OECD 301 D (Readily Biodegradability: Closed Bottlte Test) methacrylic acid attained 86 % biodegradation within 28 days. The"pass" level of 60 % was reached within 10 days of exceeding 10 % level. Therefore methacrylic acid can be termed as readily biodegradable.

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