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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study with detailed documentation
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Remarks:
EL
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM
- Source: fish farmer Branislav Settey, SKLOCHEM-AGROEKOLAB, Zvolen
- Length at study initiation: 2.0 +- 1.0 cm
- Feeding during test: none

ACCLIMATION
- Acclimation period:
held in laboratory 12 days before test
held in the water of the quality used in the test for at least 7 days.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Test temperature:
21.0 - 22.0 °C
pH:
8.12 - 8.20
Dissolved oxygen:
75.46% - 83.72% of saturation
Nominal and measured concentrations:
3300 mg/L (nominal concentration, initial)
measured concentrations: max. -0.86% of nominal
Details on test conditions:
TEST SYSTEM
- Type: closed
- Materia: glass or other chemically inert material
- Aeration: not during test
- No. of organisms per concentration: 7
- Biomass loading rate: max. 1.0 g fish/L

HOLDING WATER:
- dechlorinated tap water (left for at least 48 h in water canisters): Chlorine: < 10 µg/L, 151 mg/L (CaCO3)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO 6341 test water
- Intervals of water quality measurement: oxygen and pH measured daily

OTHER TEST CONDITIONS
- Adjustment of pH: yes, 1 N NaOH to pH 8.2 to ensure solubility of the test substance.
- Photoperiod: 16 h light / 8 h dark

EFFECT PARAMETERS MEASURED:
mortality recorded after 24, 48, 72 and 96 hours

TEST CONCENTRATIONS
- 3300 mg/L
- Range finding study: yes
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 3 300 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
no mortality observed in 96 h
Results with reference substance (positive control):
- Mortality: 96h-EC50 = 117.09 mg/L (mean of 3 determinations)
Validity criteria fulfilled:
yes
Executive summary:

The short term toxicity of TC to the fish Poecilia reticulata was tested according to OECD guideline 203 at static test conditions. No mortality was observed within 96 h at the only tested concentration of 3300 mg/L. LC50 (96 h) > 3300 mg/L.

Description of key information

The short term toxicity of TC to the fish Poecilia reticulata was tested according to OECD guideline 203. LC 50 (96 h) > 3300 mg/L.

Key value for chemical safety assessment

Additional information

The short term toxicity of TC to the fish Oncorhynchus mykiss was tested according to OECD guideline 203. No mortality was observed within 96 h at 1000 mg/L, the highest concentration tested. LC50 (96 h) > 1000 mg/L.

The short term toxicity of TC to the fish Danio rerio was tested according to OECD guideline 203. No mortality was observed within 96 h at 1000 mg/L, the highest concentration tested. LC50 (96 h) > 1000 mg/L.