6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study with detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

EL

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: own rearing, original source Water Research Institute, Bratislava, Slovak Republic
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

21.2 - 22.0 °C

pH:

8.14 - 8.20

Dissolved oxygen:

6.01 - 6.65 mg/L

Nominal and measured concentrations:

3300 mg/L (nominal concentration, initial)
measured concentrations: + 6.7%/ +16.1% of nominal at beginning/ end of test.

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 mL
- Type: closed
- Material: glass or other chemically inert material
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL/animal

- Holding water: dechlorinated tap water (Chlorine: < 10 µg/L, 151 mg/L (CaCO3))

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO 6341 test water which was aereated before use.
- Intervals of water quality measurement: oxygen and pH measured at beginning and end of test

OTHER TEST CONDITIONS
- Adjustment of pH: yes, 1 N NaOH to pH 8.2 to ensure solubility of the test substance
- Photoperiod: 16 h light / 8 h dark

EFFECT PARAMETERS MEASURED:
immobilisation recorded at 24 and 48 h

TEST CONCENTRATIONS
- 3300 mg/L
- Range finding study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 3 300 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- 48 h-EC50: 0.68 mg/L (mean of 3 determinations)

Immobilisation at 3300 mg/L: 0% after 24 h, 10% after 48 h

Validity criteria fulfilled:

yes

Executive summary:

The short term toxicity of TC to Daphnia magna was tested according to OECD guideline 202. 10% immobilisation was observed within 48 h at the only tested concentration of 3300 mg/L. Therefore, the 48h-EC50 was determined to be >3300 mg/L.

Description of key information

The 48 h short term toxicity of TC to Daphnia magna was tested according to OECD guideline 202. EC50 (immobilisation, 48 h) > 3300 mg/L.

Additional information

10% immobilisation were observed within 48 h at the only tested concentration of 3300 mg/L.