Butyryl chloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

BASF-Test: The study was conducted according to an internal BASF method which in principle is comparable to the OECD Guideline 401.
A test group consisting of 5 animals/sex was treated by single gavage application with a solution of test substance in olive oil. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 14 days, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observations period also were subjected to necropsy.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Butyryl chloride

EC Number:

205-498-5

EC Name:

Butyryl chloride

Cas Number:

141-75-3

Molecular formula:

C4H7ClO

IUPAC Name:

butanoyl chloride

Details on test material:

- Name of test material (as cited in study report): n-büttersäurechlorid
- Substance number: XXVI 622
- Substance type: liquid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gassner
- Weight at study initiation: Male mean weight: 221 g; Female mean weight: 189 g

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

Application volume 10 mg/L

Doses:

316, 464, 681, 1000, 1470, 2150, 3160, 4640 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: daily (except the weekends)
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 000 - 1 470 mg/kg bw

Based on:

test mat.

Mortality:

0/10 animals died at 316 mg/kg bw
0/10 animals died at 464 mg/kg bw
1/10 animals (female) died at 681 mg/kg bw
0/10 animals died at 1000 mg/kg bw
10/10 animals died at 1470 mg/kg bw
10/10 animals died at 2150 mg/kg bw
10/10 animals died at 3160 mg/kg bw
10/10 animals died at 4640 mg/kg bw
(deaths occurred within 1 hour)

Clinical signs:

other: Dyspnea, apathy, uncoordinated movement, atonia, tremor, tonic convulsion, clonic cramp, cyanosis, dehydration and salivation was observed at doses from 316-4640 mg/kg bw.

Gross pathology:

Animals that died during the test: heart: acute dilatation both sides, local acute hyperaemia; thorax: slight hydrothorax; lungs: emphysema; stomach: astringent surface, diffuse dark-redness, deep heomorrhagic ulcerations: bowles: astringent serosa, diffuse redness.
Animals sacrificed after the 14-day observation period: no abnormalities.