2-methylcyclohexyl acetate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

December 26, 1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 301 C(Ready Biodegradability: Modified MITI Test (I))

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

not specified

Test organisms (species):

activated sludge

Details on inoculum:

- Initial biomass concentration: 30 mg/L

Test type:

other: Readily biodegradability

Water media type:

freshwater

Limit test:

no

Total exposure duration:

28 d

Details on test conditions:

TEST SYSTEM
Test equipment: Improved type for a volatile substances

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): BOD and test item concentration by GC.

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L.

Reference substance (positive control):

not specified

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: % biodegradability

Test item 2 -methylcyclohexyl acetate was readily biodegradable under test conditions.

BOD (28 days): 99% degradation

Test material analysis by GC (28 days): 100% degradation.

Validity criteria fulfilled:

not specified

Conclusions:

The NOEC for microorganism toxicity of the test item 2-methylcyclohexyl acetate was determined to be 100 mg/L.

Executive summary:

A readily biodegradation test was performed on 2 -methylcyclohexyl acetate by MITI according to OECD Guideline 301 C. 30 mg/L of activated sludge were exposed to 100 mg/L test item for 4 weeks. The biodegradation obtained by indirect analysis was 99% (basis for effect: BOD), and by direct analysis 100% (basis for effect: GC) and therefore, 2 -methylcyclohexyl acetate was determined to be readily biodegradable. According to the current guidelines, the NOEC for microorganism toxicity was determined to be 100 mg/L since the compound degrades well in the ready biodegradability study.

Description of key information

Key study: Test method OECD 301C. No data on GLP. The NOEC (28 days) for 2-methylcyclohexyl acetate was determined to be 100 mg/L (basis for effect: biodegradation).

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

100 mg/L

Additional information

Key study: The NOEC (28 days) for 2 -methylcyclohexyl acetate was determined to be 100 mg/L since the compound degrades well (99% biodegradation by BOD analysis, 100% biodegradation by GC analysis) in a ready biodegradability test according to OECD 301 C.