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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

toxicity to microorganisms
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
2 (reliable with restrictions)

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
other: DIN EN ISO 11348-2
Principles of method if other than guideline:
A suspension of bioluminiscent bacteria is brought into a medium containing a specific concentration of copper slag extracts. After 30 minutes, the inhibition of bioluminiscence is assessed.
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Slag, copper smelting
Slag, copper smelting
Details on test material:
Slow cooled copper slag (stones) with the following chemical composition:
Fe 40.01%
SiO2 32.12 %
Cu 9300 mg/kg
Pb 4150 mg/kg
Zn 25100 mg/kg
As 12 mg/kg
Ni < 5 mg/kg
Co 310 mg/kg
Cd 17 mg/kg
Sn 1545 mg/kg

Sampling and analysis

Details on sampling:
at Tend

Test solutions

Details on test solutions:
Eluates prepared in ratio 1 /10 ( 100 g sample in 1000 ml water ) according to DIN 38414
Concentrations in eluate ( mg/l)
As 0.027
Ba 0.11
Ca 14
Cd <0.0001
Co <0.01
Cu 0.02
Hg < 0.0002
Mo 0.12
Ni < 0.01
Pb < 0.027
Sb 0.004
Tl <0.0005
Zn 0.01

Test organisms

Test organisms (species):
Vibrio fisheri

Study design

Test type:
Water media type:
Total exposure duration:
30 min

Test conditions

DOC 1.43 mg/l

Results and discussion

Effect concentrations
30 min
Dose descriptor:
other: EC20
Effect conc.:
> 80 g/L
Conc. based on:
test mat.
Basis for effect:
other: bioluminescence

Any other information on results incl. tables

Concentration of the eluate in test concentration

Eluate (1-10)

Luminescent bacteria toxicity, GI - value



*Smallest dilution factor that results in a 20% light emission reduction

EC 20

> 800 ml eluate/L


The above values can be converted to toxicity for copper slag by taking into account the amount of slag used to produce the eluate : 100 g slag / 1000 ml water

EC 20

> 80 g copper slag /L

Applicant's summary and conclusion

An EC20 value of >80 g/L was observed.
Executive summary:

Effect of water extracts of copper slags on the bioluminescence of Vibrio fischeri was assessed during a 30 min experiment. The test was performed according to DIN EN ISO 11348 -2. An EC20 value of >80 g/L was observed.