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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Toxic hazards evaluation of five atmospheric pollutants from army ammunition plants.
Author:
Kinkead ER, MacEwen JD, Haun CC, Vernot EH
Year:
1977
Bibliographic source:
US AMBRD: TR 7703, NTIS ADA043957
Reference Type:
secondary source
Title:
BUA-Stoffbericht Nr. 41: Nitrotoluole
Author:
BUA (Beratergremium umweltrelevanter Altstoffe) (BUA)
Year:
1989
Bibliographic source:
Gesellschaft Deutscher Chemiker (Hrsg Behret) Bd 41, VCH (Verlag Chemie), Weinheim

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
yes
Remarks:
Analytical purity not reported. Animal housing conditions not reported. Only one dose concentration tested
GLP compliance:
no
Remarks:
GLP was not mandatory at the time of the study
Test type:
acute toxic class method

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitrotoluene
EC Number:
202-728-6
EC Name:
3-nitrotoluene
Cas Number:
99-08-1
Molecular formula:
C7H7NO2
IUPAC Name:
1-methyl-3-nitrobenzene
Constituent 2
Chemical structure
Reference substance name:
4-nitrotoluene
EC Number:
202-808-0
EC Name:
4-nitrotoluene
Cas Number:
99-99-0
Molecular formula:
C7H7NO2
IUPAC Name:
1-methyl-4-nitrobenzene
Details on test material:
- Name of test material (as cited in study report): m-nitrotoluene
- Analytical purity: no data

Test animals

Species:
rat
Strain:
other: Sprague-Dawley and CFE rats
Sex:
male

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: air
Details on inhalation exposure:
m-nitrotoluene was tested at a saturated vapour concentration. Production of essentially saturated vapours of the m-nitrotoluene was accomplished by bubbling dry air through a fritted disc immersed in the sample. The resultant vapours were then passed through a 9-liter glass chamber containing the experimental animals.
All exposures were continuously analyzed using a Beckman Model 400 total hydrocarbon analyzer.
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
4 h
Concentrations:
157 ppm (77% saturation) = 880 mg/m³
No. of animals per sex per dose:
10
Control animals:
other: not applicable
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: All animals were observed for sings of toxicity and mortality during exposure and for the 4-days immediately following exposure. Animals were weighed immediately prior to exposure and again at 14 days postexposure on survivors
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, grosspathology
Statistics:
Determination of the LC50 was made by probit method (Finney, 1952, Probit Analysis, 2nd Edition, King Review Press)

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 880 mg/m³ air
Exp. duration:
4 h
Body weight:
All animals gained weight normally during the 14-day observation period.

Any other information on results incl. tables

No death occurred during exposure or during the subsequent 14-d observation 
period. Gross pathologic examination of rats sacrificed after  14 days revealed 
no leasions which could be attributed to exposure (no further information given.)

Applicant's summary and conclusion

Executive summary:

Kinkead ER (1977):

10 rats were exposed to an atmosphere essentially saturated with 3-nitrotoluene for four hours (rat: 157 ppm = 880 mg/m³) according to a method similar to OECD guideline 403 with deviations (Analytical purity not reported. Animal housing conditions not reported. Only one dose concentration tested). All animals were observed for signs of toxicity and mortality during exposure and for the 14-days immediately following exposure. Animals were weighed immediately prior to exposure and again at 14 days postexposure on survivors.

Determination of the LC50 was made by probit method (Finney, 1952, Probit Analysis, 2nd Edition, King Review Press).

No death occurred during exposure or during the subsequent 14 day post exposure observation period. No lesions attributable to exposure could be discovered during gross pathological evaluation, neither in rats nor in mice.

LC50 was estimated to be greater than 880 mg/m³.