Ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Data is from experimental report

Qualifier:

according to guideline

Guideline:

other: EEC Guideline - L251 Vol. 27, 84/449/EEC: C. (static procedure)

Deviations:

yes

Remarks:

No stock solution was prepared. Fish size: 19 - 28 mm (average: 23 mm).

GLP compliance:

yes

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Test system : Zebra-Fish (Brachydanio rerio)
Number of fishes: 10 fishes per concentration and control 10 fishes per aquarium
Length : 23 mm (19-28 mm)
Weight : 0.13 g (0.07-0.23 g)
Loading : 0.09 g/l
Feeding: NO
Adaptation: 24 hours / no food 24 hours prior to exposure
Acclimatisation: 33 DAYS

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

160 mg CaC03/L

Test temperature:

22±1°C

pH:

pH of test solutions including blank was within pH range of 7.9 to 8.4.

Nominal and measured concentrations:

Test concentrations (mg/1) : 3.2, 5.8, 10, 18, 32, 58 (mg/l)
Remarks (nominal) : Calculated amounts of test substance to produce the desired test concentrations were given into the water and were homogeneously distributed. The fish were then transfered into the aquaria.

Details on test conditions:

Water : Dechlorinated tap water (carbon filter)
Aeration : none
Light : Fluorescent light, 16 hours daily

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 58 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

83 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% C. I. - 44-65000 mg/1

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

33 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% C. I. - 21-93 mg/1

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

14 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% C. I. - 7.1-21 mg/l

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Graphically determined

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

5.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 58 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Controls - Mortalities in blank : 0 %

Reported statistics and error estimates:

The LC-50 values were calculated according to BERKSON, JASA 48 <1953), LC-values were graphically determined on gausso-logarithmic probability paper.

Validity criteria fulfilled:

not specified

Conclusions:

The 96 hour LC50 value of test chemical in Danio rerio was determined graphically as 12 mg/l and calculated as 14 mg/l (95% confidence limits 7.1-21 mg/l). LC0 (96 h) in test was 5.8 mg/l and LC100 (96 h) in test was >58 mg/l. Based on the LC50 (14 mg/l) chemical was consider as toxic and classifed as aquatic chronic 3.

Executive summary:

An acute toxicity to fish study was carried out for 96 hr for evaluating the effect of test chemical. The study was conducted as per EEC-Guideline L251 Vol. 27, 84/449/EEC: C. Zebra-Fish (Brachydanio rerio) of length 23 mm (19-28 mm), weight of 0.13 g (0.07-0.23 g) was used as a test organism. Test organims was acclimated for a period of 33 days. Feeding was not done during the study and fishes were also not fed with food 24 hours prior to exposure. Fishes (total 10 fish/conc.) were exposed to different concentrations (3.2, 5.8, 10, 18, 32, 58 (mg/l) of test chemical in test aquaria. Study was performed under static conditions at a temperature of 22±1°C, hardness of 160 mg/l CaC03 for 96 hrs under fluorescent light, 16 hours daily. The LC-50 values were calculated according to BERKSON, JASA 48 <1953), LC-values were graphically determined on gausso-logarithmic probability paper. No mortalities were observed in the control vessel. On the basis of effect of test chemical on mortality of the test organism, the 96 hr LC0 and LC100 value was determined to be 5.8 and > 58 mg/l, respectively. On the other hand, 96 hr median lethal concentration (LC50) was determined to be 12 mg/l determined graphically and 14 mg/l with 95% C. I. - 7.1-21 mg/l, respectively. Thus, test chemical was considered as toxic and hence, considered to be classified in 'aquatic chronic category 3' as per the CLP classification criteria.

Description of key information

An acute toxicity to fish study was carried out for 96 hr for evaluating the effect of test chemical (Experimental study report, 1989). The study was conducted as per EEC-Guideline L251 Vol. 27, 84/449/EEC: C. Zebra-Fish (Brachydanio rerio) of length 23 mm (19-28 mm), weight of 0.13 g (0.07-0.23 g) was used as a test organism. Test organism was acclimated for a period of 33 days. Feeding was not done during the study and fishes were also not fed with food 24 hours prior to exposure. Fishes (total 10 fish/conc.) were exposed to different concentrations (3.2, 5.8, 10, 18, 32, 58 (mg/l) of test chemical in test aquaria. Study was performed under static conditions at a temperature of 22±1°C, hardness of 160 mg/l CaC03 for 96 hrs under fluorescent light, 16 hours daily. The LC-50 values were calculated according to BERKSON, JASA 48 <1953), LC-values were graphically determined on gausso-logarithmic probability paper. No mortalities were observed in the control vessel. On the basis of effect of test chemical on mortality of the test organism, the 96 hr LC0 and LC100 value was determined to be 5.8 and > 58 mg/l, respectively. On the other hand, 96 hr median lethal concentration (LC50) was determined to be 12 mg/l determined graphically and 14 mg/l with 95% C. I. - 7.1-21 mg/l, respectively. Thus, test chemical was considered as toxic and hence, considered to be classified in 'aquatic chronic category 3' as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

14 mg/L

Additional information

An acute toxicity to fish study was carried out for 96 hr for evaluating the effect of test chemical (Experimental study report, 1989). The study was conducted as per EEC-Guideline L251 Vol. 27, 84/449/EEC: C. Zebra-Fish (Brachydanio rerio) of length 23 mm (19-28 mm), weight of 0.13 g (0.07-0.23 g) was used as a test organism. Test organism was acclimated for a period of 33 days. Feeding was not done during the study and fishes were also not fed with food 24 hours prior to exposure. Fishes (total 10 fish/conc.) were exposed to different concentrations (3.2, 5.8, 10, 18, 32, 58 (mg/l) of test chemical in test aquaria. Study was performed under static conditions at a temperature of 22±1°C, hardness of 160 mg/l CaC03 for 96 hrs under fluorescent light, 16 hours daily. The LC-50 values were calculated according to BERKSON, JASA 48 <1953), LC-values were graphically determined on gausso-logarithmic probability paper. No mortalities were observed in the control vessel. On the basis of effect of test chemical on mortality of the test organism, the 96 hr LC0 and LC100 value was determined to be 5.8 and > 58 mg/l, respectively. On the other hand, 96 hr median lethal concentration (LC50) was determined to be 12 mg/l determined graphically and 14 mg/l with 95% C. I. - 7.1-21 mg/l, respectively. Thus, test chemical was considered as toxic and hence, considered to be classified in 'aquatic chronic category 3' as per the CLP classification criteria.