Ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Data is from experimental source

Qualifier:

according to guideline

Guideline:

other: EEC Directive 93/32/EEC (December 1992)

GLP compliance:

yes

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

Preparation of the stock solution and dilution: The test substance was stirred in M4-medium for about 20 hours. Undisolved test substance was recoved by centrifugation (approx. 20 min about 17700 G). The result is a clear solution.
The study was performed with an aqueous extract (eluate) of the test substance.
Test concentrations :100 mg/L; 10 mg/L; 1 mg/L ; 0.1 mg/L; 0.01 mg/L

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Nominal and measured concentrations:

Test concentrations: 100 mg/L; 10 mg/L; 1 mg/L ; 0.1 mg/L; 0.01 mg/L

Details on test conditions:

Measured test parameter: Swimming ability of the daphina after 24 and 48 h; O2-content and pH-values at the begin and end of the exposure period

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

inhibition of the swimming ability

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

inhibition of the swimming ability

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

inhibition of the swimming ability

Remarks on result:

other: * highest test concentration with an effect of ≤ 10%

Concentration Effect Relationship after 48 h:

Nominal concentration [mg/L]	Number of daphnia without Inhibition (from 20)	Inhibition (%)
0 (Control)	20	0
100	0	100
10	4	20
1	20	0
0.1	20	0
0.01	20	0

Validity criteria fulfilled:

not specified

Conclusions:

The EL-values of test chemical in Daphnia magna, which cause after 48 h no (EC0) or 50% (EC50) and 100% (EC100) inhibition of the swimming ability compared to an untreated control are:
EC0 = 1 mg/l (nominal)
EC50 = 6.5 mg/l (nominal)
EC100 = 100 mg/l (nominal)

Executive summary:

An acute immobilisation test was carried out for assessing the effect of test chemical on aquatic invertebrates. The study was performed following the principles of the EEC Directive 93/32/EEC (December 1992) method. Daphnia magna Straus (Water flea) was used as a test organism. The test substance was stirred in M4-medium for about 20 hours. Undisolved test substance was recoved by centrifugation (approx. 20 min about 17700 G). The result is a clear solution. The study was performed with an aqueous extract (eluate) of the test substance. Daphnids were exposed to different test chemical conc. (0, 0.01, 0.1, 1, 10 and 100 mg/l) for 48 hrs. Swimming ability of the daphina after 24 and 48 h; O2-content and pH-values at the begin and end of the exposure period were measured. Based on the effect of test chemical on mobility of the test organism, the 48 hr EC0, EC50 and EC100 value was determined to be 1, 6.5 and 100 mg/l, respectively. Thus, test chemical was considered as toxic and hence, considered to be classified in 'aquatic chronic category 2' as per the CLP classification criteria.

Description of key information

An acute immobilisation test was carried out for assessing the effect of test chemical on aquatic invertebrates (Experimental study report. 2002). The study was performed following the principles of the EEC Directive 93/32/EEC (December 1992) method. Daphnia magna Straus (Water flea) was used as a test organism. The test substance was stirred in M4-medium for about 20 hours. Undisolved test substance was recoved by centrifugation (approx. 20 min about 17700 G). The result is a clear solution. The study was performed with an aqueous extract (eluate) of the test substance. Daphnids were exposed to different test chemical conc. (0, 0.01, 0.1, 1, 10 and 100 mg/l) for 48 hrs. Swimming ability of the daphina after 24 and 48 h; O2-content and pH-values at the begin and end of the exposure period were measured. Based on the effect of test chemical on mobility of the test organism, the 48 hr EC0, EC50 and EC100 value was determined to be 1, 6.5 and 100 mg/l, respectively. Thus, test chemical was considered as toxic and hence, considered to be classified in 'aquatic chronic category 2' as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

6.5 mg/L

Additional information

Various experimental studies of the test chemical were reviewed for short term toxicity to aquatic invertebrate end point which are summarized as below:

 

In an experimental study from study report (2002), an acute immobilisation test was carried out for assessing the effect of test chemical on aquatic invertebrates. The study was performed following the principles of the EEC Directive 93/32/EEC (December 1992) method. Daphnia magna Straus (Water flea) was used as a test organism. The test substance was stirred in M4-medium for about 20 hours. Undisolved test substance was removed by centrifugation (approx. 20 min about 17700 G). The result is a clear solution. The study was performed with an aqueous extract (eluate) of the test substance. Daphnids were exposed to different test chemical conc. (0, 0.01, 0.1, 1, 10 and 100 mg/l) for 48 hrs. Swimming ability of the daphina after 24 and 48 h; O2-content and pH-values at the beginning and end of the exposure period were measured. Based on the effect of test chemical on mobility of the test organism, the 48 hr EC0, EC50 and EC100 value was determined to be 1, 6.5 and 100 mg/l, respectively. Thus, test chemical was considered as toxic and hence, considered to be classified in 'aquatic chronic category 2' as per the CLP classification criteria.

 

Another short term toxicity to aquatic invertebrate study was carried out for assessing the effect of test chemical (2002). The study was performed following the principles of the EEC Directive 93/32/EEC (December 1992) method. Daphnia magna Straus (Water flea) was used as a test organism. The test substance was stirred in M4-medium for about 20 hours. Undisolved test substance was removed by centrifugation (approx. 20 min about 17700 G). The result is a clear solution. The study was performed with an aqueous extract (eluate) of the test substance. Daphnids were exposed to different test chemical conc. (0, 0.01, 0.1, 1, 10 and 100 mg/l) for 48 hrs. Swimming ability of the daphina after 24 and 48 h; O2-content and pH-values at the beginning and end of the exposure period were measured. Based on the effect of test chemical on mobility of the test organism, the 48 hr EC0, EC50 and EC100 value was determined to be 1, 10.8 and 100 mg/l, respectively. Thus, test chemical was considered as toxic to aquatic invertebrates at environmental relevant concentrations and hence, considered to be classified in 'aquatic chronic category 3' as per the CLP classification criteria.

 

In a supporting study, short term toxicity of test chemical was studied on invertebrates. No stock solution was prepared (Experimental study report, 1989). The test substance was added directly to the water in the tanks. Test conducted on Daphnia magna Straus 1820 obtained from CIBA-GEIGY Ltd., Facilities, Basel. Cultures of daphnia are maintained in glass vessels containing approx. 2.5 L of reconstituted water at 20 ± 1°C (water is renewed partially thrice weekly). At each renewal the daphnia are fed a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h. ≤24h before the start of the test, reproductive daphnia are separated from the young by sieving all individuals through a 800 um seive. This operation is repeated 6h before the start of the test and the young (6-≤24h of age) are retained for the test. Total 20 daphnia per concentration and control 4 replicates of 5 daphnia each were exposed with the test chemical conc. (0.0010, 0.0018, 0.0032, 0.0058, 0.010 0.018, 0.032, 0.058, 0.010 mg/l) in a test beakers covered with watch glasses. The study was performed under static conditions at hardness of 240 mg CaC03/L., temperature of 20 ± 1°C under fluorescent light of approx. 1500 lux, 16 hours daily for 24 hrs. The EC-50 values were calculated according to the maximum likelihood method, probit model (Mc Cullagh, P., Nelder, J.A., 1983: Generalized linear models, Chapman & Hall, London) EC-values were graphically determined on gausso-logarithmic probability paper. The 24 hour EC50 value of test chemical in Daphnia magna based on the effect of immobilisation was determined graphically as 0.088 mg/l and calculated as 0.15 mg/l (95% confidence limits 0.085-0.52 mg/l). The 24 hr EC0 and EC100 was evaluated to be 0.018 mg/l and >0.10 mg/l, respectively.

 

On the basis of the above results, it can be concluded that the test chemical was considered as toxic to aquatic invertebratesat environmental relevant concentrations and hence, considered to be classified in 'aquatic chronic category 2' as per the CLP classification criteria.