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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

The LC50 after 96 hours is 23 mg/L nominal in the Rainbow trout
The LC50 after 96 hours is 87 mg/L nominal in the Golden Orfe

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
23 mg/L

Additional information

The results of this study indicated that Yoracryl Yellow RL Liquid 100 was rapidly toxic to rainbow trout (oncorhynchus mykiss) at a concentration of 100 mg/L. This concentration resulted in a median period of survival of exposed fish of approximately 25 hours and killed all ten of the fish within 48 hours. The lowest concentration of the sample which resulted in 100 % mortality of exposed rainbow trout within 96 hours was 56 mg/L. A no-observed effect concentration could not be determined from this study since the lowest test concentration (10 mg/L) resulted in 20 % of the fish dying within 96 hours.

The concentration/median period of survival relationship gradient shows that over the concentration range 32 to 100 mg/L of Yoracryl Yellow RL Liquid 100, dilution significantly increased the survival time of the fish. These results are confirmed by the estimated LC50values which were 94 mg/L at 24 hours and 23 mg/L at 96 hours. The measured temperatures in the test tanks were slightly lower than the standard minimum of 14 °C at certain times during the test. The actual temperature range was 13.9 to 15.4 °C. However, this is not considered likely to have affected the response of the fish because there was no unusual pattern of mortality associated with the lower temperatures.

Basacryl Goldgelb X-GFL flüssig has been tested for acute toxicity in the Golden orfe (Leuciscus idus)

according to DIN 38 412.

The following LC50 values as nominal concentrations were determined for Basic Yellow 28 acetate:


greater 220 mg/L

1 % significance level


greater 220 mg/L

1 % significance level


greater 100 mg/L

1 % significance level


lower 220 mg/L

1 % significance level


greater 100 mg/L

10 % significance level


lower 220 mg/L

1 % significance level


about 100 mg/L



about 87 mg/L

(value of interpolation)


The following clinical signs of toxicity were noted: apathy, narcotic-like state, tumbling


No observed effect concentration: 46.4 mg/L

Maximum concentration causing no mortality: 46.4 mg/L

Minimum concentration causing 100 % mortality: 215 mg/L