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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study without detailed documentation

Data source

Reference
Reference Type:
publication
Title:
Micronucleus Test in mice on 39 Food Additives and Eight Miscellaneous Chemicals
Author:
M. Hayashi, M. Kishi, T. Sofuni, M. Ishidate
Year:
1988
Bibliographic source:
Food and Chemical Toxicology, 26(6), 487-500

Materials and methods

Type of assay:
micronucleus assay

Test material

Constituent 1
Reference substance name:
L-Cysteine, hydrochloride
IUPAC Name:
L-Cysteine, hydrochloride
Constituent 2
Reference substance name:
Cysteine hydrochloride
EC Number:
200-157-7
EC Name:
Cysteine hydrochloride
Cas Number:
52-89-1
IUPAC Name:
cysteine hydrochloride

Test animals

Species:
mouse
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
Saline
Frequency of treatment:
1 dose
Post exposure period:
24h
Doses / concentrationsopen allclose all
Remarks:
Doses / Concentrations:
125 mg/kg
Basis:

Remarks:
Doses / Concentrations:
250 mg/kg
Basis:

Remarks:
Doses / Concentrations:
500 mg/kg
Basis:

No. of animals per sex per dose:
6
Control animals:
yes, concurrent vehicle
Positive control(s):
Mitomycin C

Results and discussion

Additional information on results:
No mortality

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative
Cystein Hydrochloride is negative in an in vivo micronucleus test in mice under the consditions described in the publication.