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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20.-23.08.1962
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1962
Report date:
1962

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Method: Before OECD Guideline 405 was established, eye irritation was tested using an internal method (BASF test). White Vienna rabbits were used. 50 µL of the test substance were applied to the conjunctival sac of one eye in 2 animals. The non- or saline-treated adjacent eye served as control. The animals were observed several times on the day of treatment and up to 7 or 8 days afterwards. Findings were recorded on working days.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Isobutyl acrylate
EC Number:
203-417-8
EC Name:
Isobutyl acrylate
Cas Number:
106-63-8
Molecular formula:
C7H12O2
IUPAC Name:
isobutyl acrylate
Specific details on test material used for the study:
- Name of test material: Isobutyl acrylate, stabilized with 0.006% Hydrochinonmonomethylether

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 2.11 kg and 2.29 kg

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: adjacent eye treated with NaCl
Amount / concentration applied:
TEST MATERIAL
- Amount applied: 0.05 mL
- Concentration: undiluted
Duration of treatment / exposure:
not applicable because substance remained in the eye and was not washed out
Observation period (in vivo):
4 days
Number of animals or in vitro replicates:
2
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing: not rinsed

SCORING SYSTEM:
The original scoring system was converted into the OECD Draize score.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
24/48/72 h
Score:
0.33
Max. score:
3
Reversibility:
fully reversible within: 48 hours
Irritation parameter:
chemosis score
Basis:
animal #1
Time point:
24/48/72 h
Score:
0.33
Max. score:
4
Reversibility:
fully reversible within: 48 hours
Irritation parameter:
cornea opacity score
Basis:
animal #1
Time point:
24/48/72 h
Score:
0.33
Max. score:
4
Reversibility:
fully reversible within: 48 hours
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
24/48/72 h
Score:
0.33
Max. score:
3
Reversibility:
fully reversible within: 48 hours
Irritation parameter:
chemosis score
Basis:
animal #2
Time point:
24/48/72 h
Score:
0
Max. score:
4
Reversibility:
fully reversible within: 24 hours
Irritation parameter:
cornea opacity score
Basis:
animal #2
Time point:
24/48/72 h
Score:
0
Max. score:
4
Reversibility:
fully reversible within: 24 hours
Irritant / corrosive response data:
1 and 24 hours after instillation of the test substance into the eyes, slight conjunctivitis (Draize grade 1), slight edema (grade 1) and slight corneal opacity (grade 1) were noted. These effects were all completely reversible within 8 days.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met