3-chloro-p-toluidine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: scientifically acceptable and sufficinet documented

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Principles of method if other than guideline:

Undiluted 3-chloro-p-toluidine was applied on the shaved belly skin of rats for 4 hours in doses of 100 to 1000 µl/kg (117 to 1170 mg/kg). After 4 hours the substance was removed with water and soap. The animals were observed for 7 days.

GLP compliance:

not specified

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

95-75-9

IUPAC Name:

95-75-9

Constituent 2

Reference substance name:

3-chloro-p-toluidine

EC Number:

202-446-3

EC Name:

3-chloro-p-toluidine

Cas Number:

95-74-9

Molecular formula:

C7H8ClN

IUPAC Name:

3-chloro-4-methylaniline

Test material form:

other: liquid

Details on test material:

technically pure, undiluted 3-chloro-p-toluidine

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Administration / exposure

Type of coverage:

not specified

Vehicle:

unchanged (no vehicle)

Duration of exposure:

4 hours

Doses:

100 µl (= 117 mg)/kg bw, 250 µl (= 292.5 mg/kg bw), 500 µl (= 585 mg/kg bw), 600 µl (= 702 mg/kg bw), 800 µl (= 936 mg/kg bw), 1000 µl (= 1170 mg/kg bw).

No. of animals per sex per dose:

5 rats (100 µl), 5 rats (250 µl), 10 rats (500 µl), 10 rats (600 µl), 10 rats (800 µl), 5 rats (1000 µl).

Control animals:

no

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 765 mg/kg bw

Based on:

test mat.

Any other information on results incl. tables

From 2 to 6 hours after application of the test substance,typical signs of intoxication (cyanosis, laboured breathing, sedation) were observed. The highest dose without findings was 100 µl (117 mg/kg bw) and the lowest lethal dose was 600 µl (702 mg/kg bw).

Applicant's summary and conclusion

Executive summary:

Undiluted 3-chloro-p-toluidine was applied on the shaved belly skin of rats for 4 hours in doses of 100 to 1000 µl/kg (117 to 1170 mg/kg bw). After 4 hours the substance was removed with water and soap. The animals were observed for 7 days.

LD50 (4 hours exposure) = 650 µl 3-chloro-p-toluidine/kg bw (= ca. 765 mg/kg bw).