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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

The EC50 for the green algae Pseudokirchneriella subcapitata based on growth rate was determined to be > 10 mg/L loading rate and the EC10 4.9mg/L after exposure 72 h under static conditions, indicating a low toxicity of the test organisms to the test item.

Key value for chemical safety assessment

Additional information

One reliable guideline compliant studies on the toxicity to aquatic algae and cyanobacteria is available.

In a 72 hour acute toxicity study (2007), the cultures of the green algal species Pseudokirchneriella subcapitata were exposed to the test item under static conditions in accordance with OECD guideline 201. Due to its low solubility in water the test item was tested by preparing Water-Accommodated-Fractions (WAFs). The loading rates of 0, 0.032, 0.1, 0.32,1.0,3.2, 10 mg/L was tested. The NOEC, EC10 and EC50 values based on the loading rates on growth rate were 3.2, 4.9 and >10 mg/L, respectively.  The NOEC, EC10 and EC50 values based on loading rates on Yield were 3.2, 3.5 and 7.1 mg/L, respectively.

A second non-GLP study without analytics is disregarded due to the limited documentation.

The EC10 based on growth rate has been used for the PNEC derivation since it is the most sensitiv chronic value out of two studies.

According to the Guidance on information requirements and chemical safety assessment Chapter R.10: Characterisation of dose [concentration]-response for environment "an EC10 for a long-term test which is obtained using an appropriate statistical method (usually regression analysis) will be used preferentially. [...] There has been a recommendation within OECD in 1996 to phase out the use of the NOEC, in particular as it can correspond to large and potentially biologically important magnitudes of effect. The advantage of regression method for the estimation of ECx is that information from the whole concentration-effect relationship is taken into account and that confidence intervals can be calculated. These methods result in an ECx, where x is a low effect percentile (e.g. 5-20%). It makes results from different experiments more comparable than NOECs." Therefore, the EC10 instead of the NOEC has been used to derive the PNEC.