3,4-dichlorobut-1-ene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Adopted according to OECD SIDS (public available peer reviewed source). The original source is not available and has not been reviewed.

Data source

Reference

Reference 1

Reference Type:

secondary source

Title:

SIDS Initial Assessment Report for 11th SIAM, UNEP Publications

Author:

OECD SIDS

Year:

2003

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1967

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

An acute single dose inhalation toxicity test was performed on 6 male rats in order to determine the LC50.

GLP compliance:

no

Remarks:

GLP was not mandatory at the time of the study

Test type:

other: Single Dose Inhalation Toxicity Test

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dichlorobut-1-ene

EC Number:

212-079-0

EC Name:

3,4-dichlorobut-1-ene

Cas Number:

760-23-6

Molecular formula:

C4H6Cl2

IUPAC Name:

3,4-dichlorobut-1-ene

Details on test material:

- Name of test material (as cited in study report): 3,4-Dichlorobut-1-ene
- Analytical purity: 98%

Test animals

Species:

rat

Strain:

other: ChR;CD (SD)

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 246-295 g bw

Administration / exposure

Route of administration:

inhalation: vapour

Vehicle:

not specified

Details on inhalation exposure:

The test material was metered by a syringe drive into a stainless steel T tube where it was vaporized at 120-150°C. The resulting vapours were carried by a stream of air into a 16 liter exposure chamber.

Analytical verification of test atmosphere concentrations:

yes

Remarks:

The chamber atmosphere was analyzed at least once every hour by a gas chromatographic method.

Duration of exposure:

4 h

Concentrations:

1000, 2250, 3000 ppm

No. of animals per sex per dose:

6 (only male animals were tested)

Control animals:

other: not necessary

Details on study design:

- Necropsy of survivors performed: yes
- Organs examined at necropsy: kidney, liver, trachea, lung, brain, testes, bone marrow, spleen, thymus, gastro intestinal tract were examined.
- Other examinations performed: clinical signs, histopathology.

Statistics:

Statistical analysis by metod of Litchfield, J. T., Jr., and F. Wilcoxon, J. Pharmacol. And Exper. Ther., 96, 99 (1949).

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

10.89 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

Deaths occurred 2-6 days post-exposure.

Clinical signs:

other: Clinical signs of irregular breathing, incoordination, unresponsiveness and lacrimation (only at the beginning of the exposure) were observed.

Body weight:

No data

Gross pathology:

No data

Other findings:

Inhalation at both high and low dosage levels was followed by degenerative lesions of the livers and/or kidneys of rats which died or killed within two days of exposure. Observable lesions were not present in the tissues examined from rats killed at seven days following low level exposure or fourteen days following medium level exposure.

Any other information on results incl. tables

On the basis of the results, 3,4 -dichlorobut-1 -ene is to be classified as Xn, R20 (Harmful by inhalation), according to the DSD classification criteria and Acute Tox. 4 (Harmful by inhalation) according to CLP classification criteria.