Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Repeated dose toxicity: inhalation

Currently viewing:

Administrative data

Endpoint:
repeated dose toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: MIGRATED DATASET. Original reference not yet available.

Data source

Reference
Title:
No information
Author:
Du Pont, Haskell Laboratory for Toxicology and Industrial|Medicine: Inhalation studies - two-week subacute, Report No.|364-75 (1975), NTIS/OTS 0533725

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Method: No data
GLP compliance:
no
Remarks:
GLP was not mandatory at the time of the study

Test material

Constituent 1
Chemical structure
Reference substance name:
3,4-dichlorobut-1-ene
EC Number:
212-079-0
EC Name:
3,4-dichlorobut-1-ene
Cas Number:
760-23-6
Molecular formula:
C4H6Cl2
IUPAC Name:
3,4-dichlorobut-1-ene
Details on test material:
- Name of test material (as cited in study report): 3,4-dichlorobut-1-ene
- Analytical purity: 98.74%

Test animals

Species:
rat
Strain:
other: ChR-CD and Long-Evans
Sex:
male

Administration / exposure

Route of administration:
inhalation: vapour
Details on inhalation exposure:
Liquid 3,4-DCB-1 was delivered at a constant rate by a syringe pump into a heated (~ 110° C) round-bottom flask. Air metered into the flask carried the resulting vapours into a glass 30-liter chamber containing the test animals. Samples of the chamber atmosphere were taken at hourly intervals during exposure by trapping 3,4-DCB-1 in hexane and analyzing the resulting mixture by gas chromatography. Half of the animals were sacrificed and autopsied at 1 day after the last exposure; the remaining animals were sacrificed after the recovery period.
Duration of treatment / exposure:
2 weeks
Frequency of treatment:
6 h/d, 5 d/w
Doses / concentrations
Remarks:
Doses / Concentrations:
20 or 200 ppm (104 or 1037 mg/m³)
Basis:

Control animals:
yes
Details on study design:
Post-exposure period: 14 days

Results and discussion

Results of examinations

Details on results:
200 ppm appeared to have a slight effect on both ChR-CD and Long-Evans rats. This was evidenced by an increased liver-to-body weight ratio and changes in liver cell morphology. These effects were not present in the ChR-CD rats after a 14-day recovery period, but the increased liver-to-body weight did persist in 2/3 Long-Evans rats 14 days post-exposure.

Effect levels

Dose descriptor:
NOAEL
Effect level:
20 ppm
Sex:
male

Target system / organ toxicity

Critical effects observed:
not specified

Applicant's summary and conclusion