Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
9/6/94 - 7/7/94
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study conducted in compliance with GLP regulations

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
1994

Materials and methods

Test guideline
Guideline:
other: OECD Guideline 301 A (new version) (Ready Biodegradability: DOC Die Away Test)
Principles of method if other than guideline:
Additional replicate run as part the DOC die away test to determine the inhibition of the inoculum is used to assess the toxicity of the submission item towards microorganisms.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4,5-dihydroxy-1,3-dimethylimidazolidin-2-one
EC Number:
223-496-2
EC Name:
4,5-dihydroxy-1,3-dimethylimidazolidin-2-one
Cas Number:
3923-79-3
Molecular formula:
C5H10N2O3
IUPAC Name:
4,5-dihydroxy-1,3-dimethylimidazolidin-2-one
Details on test material:
- Name of test material (as cited in study report): Fixapret NF
- Physical state: liquid
- Analytical purity: 54,6 %

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from a laboratory wastewater treatment plants fed with municipal and synthetic sewage
- Concentration of sludge: 30 mg/l

Study design

Test type:
static
Total exposure duration:
28 d

Results and discussion

Effect concentrations
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
ca. 100 mg/L
Conc. based on:
test mat.
Basis for effect:
other: microbial metabolism
Details on results:
Additional replicate run as part the DOC die away test to determine the inhibition of the inoculum is used to assess the toxicity of the submission item towards microorganisms.
20 mg/L DOC of test material (corresponding to approx. 108 mg/L test substance) did not have any inhibitory effect on the activity of activated sludge.

Applicant's summary and conclusion