o-phenylenediamine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed according to current OECD-Guideline and GLP, but toxicity control is missing

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

o-phenylenediamine

EC Number:

202-430-6

EC Name:

o-phenylenediamine

Cas Number:

95-54-5

Molecular formula:

C6H8N2

IUPAC Name:

benzene-1,2-diamine

Details on test material:

> 99.9 %
stability 1-5000 mg/l for 3 days given (dark, without O2-access)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, industrial, adapted

Details on inoculum:

mixture of sewage from the sewage plant of Hoechst AG, Hoechst and the sewage plant Kelsterbach 1:1
Pretreatment was done according to EG law (1.6.1.3.)
The dry weight of the sludge suspension showed 9.526 g dry substance per liter
Therefore the amount in the test has been 571,6 mg/l dry substance and within the 2,5-4 times used C-content (calculated 207,3 mg C/l

Duration of test (contact time):

47 d

Initial test substance concentration

Initial conc.:

627 mg/L

Based on:

other: CSB

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

other:

Details on study design:

parent solution: 2 g/l weigh in, homogenisation by Ultra-Turrax approx 5 min,
pH 7.4
stirred for 4 h at 21 °C - pH afterwards 7.3
decant as clear solution
parent solution 4013 mg O2/l
base concentration 627 mg/l
Room temperature 20 - 25 °C

Reference substance

Reference substance:

diethylene glycol

Remarks:

parent solution 20 g/l, pH 7.0

Results and discussion

% Degradation

% Degradation 1

Parameter:

other: CSB

Value:

< 20

Sampling time:

3 h

% Degradation 2

Parameter:

other: CSB

Value:

44

Sampling time:

7 d

% Degradation 3

Parameter:

other: CSB

Value:

72

Sampling time:

21 d

% Degradation 4

Parameter:

other: CSB

Value:

83

Sampling time:

30 d

% Degradation 5

Parameter:

other: CSB

Value:

93

Sampling time:

47 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

o-Phenylendiamine is potentially biological degradable
Under the test conditions described, the substance is good degradable.

Executive summary:

o-Phenylendiamine is potentially biological degradable Under the test conditions described, the substance is good biodegradable.