Registration Dossier

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study performed according to current OECD-Guideline and GLP, but toxicity control is missing

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1992

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
o-phenylenediamine
EC Number:
202-430-6
EC Name:
o-phenylenediamine
Cas Number:
95-54-5
Molecular formula:
C6H8N2
IUPAC Name:
benzene-1,2-diamine
Details on test material:
> 99.9 %
stability 1-5000 mg/l for 3 days given (dark, without O2-access)

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, industrial, adapted
Details on inoculum:
mixture of sewage from the sewage plant of Hoechst AG, Hoechst and the sewage plant Kelsterbach 1:1
Pretreatment was done according to EG law (1.6.1.3.)
The dry weight of the sludge suspension showed 9.526 g dry substance per liter
Therefore the amount in the test has been 571,6 mg/l dry substance and within the 2,5-4 times used C-content (calculated 207,3 mg C/l
Duration of test (contact time):
47 d
Initial test substance concentration
Initial conc.:
627 mg/L
Based on:
other: CSB
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
other:
Details on study design:
parent solution: 2 g/l weigh in, homogenisation by Ultra-Turrax approx 5 min,
pH 7.4
stirred for 4 h at 21 °C - pH afterwards 7.3
decant as clear solution
parent solution 4013 mg O2/l
base concentration 627 mg/l
Room temperature 20 - 25 °C
Reference substance
Reference substance:
diethylene glycol
Remarks:
parent solution 20 g/l, pH 7.0

Results and discussion

% Degradationopen allclose all
Parameter:
other: CSB
Value:
< 20
Sampling time:
3 h
Parameter:
other: CSB
Value:
44
Sampling time:
7 d
Parameter:
other: CSB
Value:
72
Sampling time:
21 d
Parameter:
other: CSB
Value:
83
Sampling time:
30 d
Parameter:
other: CSB
Value:
93
Sampling time:
47 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable
Conclusions:
o-Phenylendiamine is potentially biological degradable
Under the test conditions described, the substance is good degradable.
Executive summary:

o-Phenylendiamine is potentially biological degradable Under the test conditions described, the substance is good biodegradable.

Categories Display