o-phenylenediamine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline but without GLP and deficiencies in documentation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Principles of method if other than guideline:

The test was done due to the text of the method proposal (F.1.1.) "Letale Wirkung beim Zebrabärbling Brachydanio rerio" (LC0, LC50, LC100; 48-96 h) of the Umweltbundesamt, dated 1982-06-01

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

o-phenylenediamine

EC Number:

202-430-6

EC Name:

o-phenylenediamine

Cas Number:

95-54-5

Molecular formula:

C6H8N2

IUPAC Name:

benzene-1,2-diamine

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

body size 30 +/- 5 mm
10 animals per 3,5 l water

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

without feeding

Post exposure observation period:

no data

Test conditions

Hardness:

3,5 l conditioned, via activated charcoal and a ion exchanger for Cu-removal sofetened drinking water with a Ca/Mg-range of 4:1 and a hardness of approx. 15 ° dH, at pH 7.0 +/- 0.2

Test temperature:

23 +/- 2 °C

pH:

7.0 +/- 0.2, after adding test material pH was 7.7

Dissolved oxygen:

air ventilation was done with 3 bubbles per second

Salinity:

Ca/Mg-range of 4:1 and a hardness of approx. 15 ° dH, at pH 7.0 +/- 0.2

Nominal and measured concentrations:

test substance added was 1000 mg/l

Details on test conditions:

size of test vessels: 8.4 l.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

18 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

24 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

32 mg/L

Basis for effect:

mortality (fish)

Details on results:

LC0, LC50 and LC100 are statistically calculated.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 of the test item determined in Brachydanio rerio was statistically calculated with 24 mg/l after 96 h.

Executive summary:

The LC50 of the test item determined in Brachydanio rerio was statistically calculated with 24 mg/l after 96 h.