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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to current OECD-Guideline and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
Potassiumdichromat was used instead of 3,5-Dichlorophenol as a reference substance.
Principles of method if other than guideline:
.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
o-phenylenediamine
EC Number:
202-430-6
EC Name:
o-phenylenediamine
Cas Number:
95-54-5
Molecular formula:
C6H8N2
IUPAC Name:
benzene-1,2-diamine

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
100 mg/l
180 mg/l
320 mg/l
580 mg/l
1000 mg/l

Test organisms

Test organisms (species):
anaerobic bacteria from a domestic water treatment plant
Details on inoculum:
activated, aerobic sludge from of communally clarification plant, Hildesheim
pre-treatment: After sedimentation two times washed with dechlorinated tap water (autoklaviated)
culture medium: synthetical effluent according OECD 209
activated sludge dry weight: 1.379 g/l
control: culture medium with Inoculum

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
22 °C
Reference substance (positive control):
yes
Remarks:
potassiumdichromat Fa Merck

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
580 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC 80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
EC 10 = < 100 mg/l oPD
EC 20 = < 100 mg/l oPD
P95 % not to been identified

test concentration respirationsrate inhibition (%)
1000 12.6 53
580 13.4 50
320 14.4 46
180 15.8 41
100 16.8 38
Referencesubstance
60 13.2 51
Control 1
- 25.8 -
Control 2
- 28.0 -
difference of control respiration rates was < 15 %
Results with reference substance (positive control):
With Potassiumdichromate at a concentration of 60 mg/l test medium, inhibition was 51 % .

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of o-Phenylenediamine was 580 mg/l.

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