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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to current OECD-Guideline and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
Potassiumdichromat was used instead of 3,5-Dichlorophenol as a reference substance.
Principles of method if other than guideline:
.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
o-phenylenediamine
EC Number:
202-430-6
EC Name:
o-phenylenediamine
Cas Number:
95-54-5
Molecular formula:
C6H8N2
IUPAC Name:
benzene-1,2-diamine

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
100 mg/l
180 mg/l
320 mg/l
580 mg/l
1000 mg/l

Test organisms

Test organisms (species):
anaerobic bacteria from a domestic water treatment plant
Details on inoculum:
activated, aerobic sludge from of communally clarification plant, Hildesheim
pre-treatment: After sedimentation two times washed with dechlorinated tap water (autoklaviated)
culture medium: synthetical effluent according OECD 209
activated sludge dry weight: 1.379 g/l
control: culture medium with Inoculum

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
22 °C
Reference substance (positive control):
yes
Remarks:
potassiumdichromat Fa Merck

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
580 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC 80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
EC 10 = < 100 mg/l oPD
EC 20 = < 100 mg/l oPD
P95 % not to been identified

test concentration respirationsrate inhibition (%)
1000 12.6 53
580 13.4 50
320 14.4 46
180 15.8 41
100 16.8 38
Referencesubstance
60 13.2 51
Control 1
- 25.8 -
Control 2
- 28.0 -
difference of control respiration rates was < 15 %
Results with reference substance (positive control):
With Potassiumdichromate at a concentration of 60 mg/l test medium, inhibition was 51 % .

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of o-Phenylenediamine was 580 mg/l.