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EC number: 237-511-5 | CAS number: 13822-56-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-04-19 to 1994-04-20
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The test procedure was described in sufficient detail and was in accordance with generally accepted scientific standards, with acceptable restrictions. The restrictions were that a reference substance was not used to check the sensitivity of the microorganisms. The study was conducted in compliance with GLP.
- Principles of method if other than guideline:
- Method: Oxygen consumption test (Huls method)
- GLP compliance:
- yes
- Vehicle:
- no
- Details on test solutions:
- Test substance was dissolved in water to give a stock solution of 50 mg/ml. This was pipetted in graded concentrations directly in the test flasks.
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- The bacterial culture was originally from the Institute for Water, Soil, and Air Hygiene of the BGA (Berlin).
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 5.75 h
- Test temperature:
- 24.5-26.1 °C
- Nominal and measured concentrations:
- 12.5, 25, 37.5 and 50 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml BOD bottles
- Type (delete if not applicable): closed
- No. of vessels per concentration (replicates): 4 with test substance (2 sterilised with HgCl2).
- No. of vessels per control (replicates): 4 without test substance and sterilised with HgCl2. 5 flasks without test substance or HgCl2.
- Biomass loading rate: 10 E+05 bacteria count/ml
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Oxygen concentration measured at end of test.
TEST CONCENTRATIONS
- Range finding study was used to determine the concentrations used in the main study - Reference substance (positive control):
- no
- Key result
- Duration:
- 5.75 h
- Dose descriptor:
- EC10
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 5.75 h
- Dose descriptor:
- EC50
- Effect conc.:
- 43 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- A positive control was not included.
- Reported statistics and error estimates:
- Regression analysis performed on results
- Conclusions:
- A pseudomonas putida respiration inhibition 5.75 hour EC50 of 43 mg/l and EC10 of 13 mg/l were determined in a reliable study described in sufficient detail and conducted in accordance with generally accepted scientific standards, with acceptable restrictions. The restrictions were that a reference substance was not used to check the sensitivity of the microorganisms. The study was conducted in compliance with GLP.
Reference
Table 1Effect of test substance on oxygen consumption
Conditions |
Concentration of test substance (mg/ml) |
0 |
12.5 |
25 |
37.5 |
50 |
Test substance with HgCl2 |
Initial value* |
8.24 |
8.28 |
8.22 |
8.14 |
8.17 |
Difference** |
|
-0.04 |
0.02 |
0.11 |
0.08 |
|
Test substance without HgCl2 |
Final value* |
4.25 |
4.60 |
5.52 |
6.01 |
6.30 |
Oxygen consumption after incubation |
|
3.99 |
3.69 |
2.7 |
2.15 |
1.86 |
Absolute difference after incubation |
|
|
0.30
|
1.29 |
1.84 |
2.31 |
% effect |
|
|
7.6 |
32.3 |
46.1 |
53.5 |
* Average of 2 (or four for control) cultures
** Difference between control and test values
Linear regression after Probit transformation
Measure value mg/ml |
% effect |
log 10 |
Probit |
Expected Probit |
12.5 |
7.6 |
1.0969 |
3.6245 |
3.7 |
25 |
32.3 |
1.3979 |
4.5442 |
4.4 |
37.5 |
46.1 |
1.6740 |
4.9024 |
4.9 |
50 |
53.5 |
1.6990 |
5.0876 |
5.2 |
Regression analysis: Log Concentration = a; Probit = y; y=a+bx
a = 0.98
b= 2.47
n= 4
r =0.9898
Description of key information
Toxicity to microorganisms: 5.75 hour EC10 13 mg/L (respiration inhibition test) read-across from an analogous substance
Key value for chemical safety assessment
- EC50 for microorganisms:
- 43 mg/L
- EC10 or NOEC for microorganisms:
- 13 mg/L
Additional information
No reliable study of respiration inhibition of activated sludge is available for the submission substance, 3-(trimethoxysilyl)propylamine (CAS No. 13822-56-5), therefore good quality data for an analogous substance, (3-aminopropyl)triethoxysilane (CAS No. 919-30-2) have been read-across. Both substances hydrolyse rapidly and share the same silanol hydrolysis product, 3-aminopropylsilanetriol. The other hydrolysis products are methanol and ethanol respectively.
Ethanol and methanol do not have adverse effects on microorganisms (OECD 2004a and OECD 2004b).
In a respiration inhibition test in a single bacteria strain (Pseudomonas putida) study conducted on (3-aminopropyl)triethoxysilane, 5.75 h EC50 of 43 mg/L and EC10 of 13 mg/L were determined. The study was considered to be reliable and conducted in accordance with generally accepted scientific standards and described in sufficient detail and in compliance with GLP with acceptable restrictions. The restrictions were that a reference substance was not used to check the sensitivity of the microorganisms. To support this result, for triethoxy(3-isocyanatopropyl)silane (CAS No. 24801-88-5), an activated sludge respiration inhibition 3 h NOEC value of 10 mg/L was reported in accordance with OECD 209.
3-(Trimethoxysilyl)propylamine (CAS No. 13822-56-5) and (3-aminopropyl)triethoxysilane (CAS No. 919-30-2) are within an analogue group of substances within which in general, there is some evidence of toxicity to microorganisms i.e. an effect concentration (EC) could be derived and was <1000 mg/L.
The table below presents microorganism toxicity data available for substances within the analogue group III-8 of chlorosilanes, siloxanes, silanes and alkoxysilanes etc. with amino group(s) in side chain after any very rapid hydrolysis has been accounted for. 3-(Trimethoxysilyl)propylamine (CAS No. 13822-56-5) and 3-aminopropyltriethoxysilane (CAS No. 919-30-2) are members of this analogue group.
It is therefore considered valid to read-across the results for (3-aminopropyl)triethoxysilane (CAS No. 919-30-2) to fill the data gap for the registered substance. Additional information is given in a supporting report (PFA 2013l) attached in Section 13.
Table: Microorganism toxicity data available for substances within the relevant analogue group III-8 of chemicals
CAS |
Chemical Name (CAS Name) |
Guideline Number |
Test method |
Species |
Duration |
Result: E(I)C50 (mg/l) |
Result: NOEC (Or EC10/EC20) (mg/l) |
Klimisch code |
27445-54-1 |
Cyclohexanamine,N-[(diethoxymethylsilyl)methyl]- |
OECD 209 |
ASRI |
|
3hr - but stirred 24hr |
624.2 mg/l (EC20 240.2 mg/l) |
|
1 |
3068-78-8 |
N-[3-(trimethoxysilyl)propylcyclohexylamine] |
OECD 209 |
ASRI |
|
3 h |
>1000 |
EC20 = 203 mg/l |
1 |
157923-74-5 |
2,2-dimethyl-4-(trimethoxysilyl)butylamine |
OECD 209 |
ASRI |
|
3 h |
>1000 |
EC15 869. 17.5% inhibition at 1000 mg/l. |
1 |
227085-51-0 |
N-ethyl-3-trimethoxysilyl-2-methylpropanamine |
OECD 209 |
ASRI |
|
3 h |
>1000 |
1 |
|
919-30-2 |
3-aminopropyltriethoxysilane |
Huls AG method |
oxygen consumption |
P. putida |
5.75 h |
43 mg/l |
EC10 13 mg/l |
2 |
82985-35-1 |
Bis(trimethoxysilylpropyl)amine |
OECD 209 |
ASRI |
|
3 h |
estimated value, approximately 1000 |
220 Nitrification control indicated that heterotrophic inhibition of respiration rate was lower than the total inhibition. |
1 |
1760-24-3 |
N-(3-(trimethoxysilyl)propyl)ethylenediamine |
DIN 38 412, Part 8 (Pseudomonas cell multiplication inhibition test) |
growth inhibition test |
P. putida |
16 h |
67 mg/l |
EC10 25 mg/l |
1 |
[N,N-Bis(trimethylsilyl)aminopropyl]methyldiethoxysilane |
OECD 209 |
ASRI |
3 h |
>100 |
1 |
|||
None19 |
reaction mix (reaction mass of 6 benzylated amine silanes) |
OECD 209; EC C.11 |
ASRI |
3 h |
569 |
130; EC20 172 |
1 |
An IC50 value of 3400 mg/L for 3-(trimethoxysilyl)propylamine (CAS No. 13822-56-5) was reported in secondary literature (handbook). The original reference was not available for review and no further information is available. The reliability of this result is not assignable.
References:
OECD (2004a): SIDS Initial Assessment Report for SIAM 19, Berlin, Germany, 18-20 October 2004, Methanol, CAS 67-56-1.
OECD (2004b): SIDS Initial Assessment Report for SIAM 19, Berlin, Germany, 19-22 October 2004, Ethanol, CAS 64-17-5.
PFA (2013l). Peter Fisk Associates, STP Microorganism toxicity III-8 Analogue Group report, PFA.300.003.008
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