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Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1959
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given. Meets basic scientific principles.

Data source

Reference
Reference Type:
publication
Title:
The Toxicological Basis of Threshold Limit Values: 5. The Experimental Inhalation of Vapor Mixtures by Rats, with Notes upon the relationship between single dose inhalation and single dose oral data.
Author:
Pozzani, U.C., Weil, C.S. and Carpenter, C.P.
Year:
1959
Bibliographic source:
Industrial Hygiene Journal, 20:364-369.

Materials and methods

Principles of method if other than guideline:
Study preceded guidelines. Principles of method similar to those of guideline studies.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Isopropyl acetate
EC Number:
203-561-1
EC Name:
Isopropyl acetate
Cas Number:
108-21-4
Molecular formula:
C5H10O2
IUPAC Name:
isopropyl acetate
Details on test material:
- Name of test material (as cited in study report): Isopropyl Acetate

Test animals

Species:
rat
Strain:
other: Carworth Farms-Nelson
Sex:
female

Administration / exposure

Route of administration:
inhalation: vapour
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
The vapor was produced when liquid chemical was delivered by a syringe pump into a heated evaporator through which metered air was delivered to generate the desired concentration. The resultant vapors were flushed into a 9-liter dessicator which served as an inhalation chamber for six rats. For dual exposures a bleeder rotameter was necessary to provide equal airflows containing equal concentrations of components A and B to the desiccator from each vaporizing system. For example, if the airflow containing 15 mg/liter of component A was 3 liters/min, and the airflow containing 15 mg/liter of component B was 4 liters/min, then one liter/min of the latter flow would be discharged through the bleeder rotameter.
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
8 h
Concentrations:
No data
No. of animals per sex per dose:
6
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
Statistics:
The LC50 value, which represents the vapor concentration in mg/liter which would be expected to kill half of the animals, was calculated by the method of moving averages for each commercial mixture and pair. The LC50 values of the individual components were determined and these were used in an equation described by Finney to predict the LC50 values of the vapor mixtures or pairs in which they occurred.

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
LC50
Effect level:
50 600 mg/m³ air
Exp. duration:
8 h

Any other information on results incl. tables

The reported range for the LC50 value was 43,000 – 59,500 mg/m3. One ppm equals 4.18 mg/m3.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Executive summary:

Pozzani et al (1959) reported an 8 -hr inhalation LC50 value of 50,600 mg/m3 for isopropyl acetate vapor in female rats.