Dimethyl carbonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

5 December 2000 to 7 December 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 and 100 mg/L
- Sampling method: samples "drawn up" from containers
- Sample storage conditions before analysis: 0 hour samples taken immediately after preparation

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: weighed amount (20 mg) of dimethylcarbonate were added to 200 mL of reconstituted water in order to obtain the final nominal concentration of 100 mg/L
- Eluate:
- Differential loading:
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
- Concentration of vehicle in test medium (stock solution and final test solution):
- Evidence of undissolved material (e.g. precipitate, surface film, etc):

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: not stated
- Strain: not stated
- Source: bred in the laboratory
- Age at study initiation (mean and range, SD): not more than 24 h
- Weight at study initiation (mean and range, SD): not stated
- Length at study initiation (length definition, mean, range and SD): not stated
- Valve height at study initiation, for shell deposition study (mean and range, SD): not stated
- Peripheral shell growth removed prior to test initiation: not stated
- Method of breeding: not stated
- Feeding during test
- Food type: green algae (Selenastrum capricornutum)
- Amount: not stated
- Frequency: daily (animals not fed during treatment period)


ACCLIMATION
- Acclimation period: none - animals cultured in water similar to treatment water
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):


QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

Less than 250 mg/L calcium carbonate

Test temperature:

20/21C

pH:

7.6/7.9

Dissolved oxygen:

8.6/9.0 mg/L dissolved oxygen

Salinity:

not measured

Nominal and measured concentrations:

Nominal concentations: 0 and 100 mg/L

Time h Nominal concentration DMC mg/L Measured concentration DMC mg/L
0 100 82.29
48 without Daphniae 100 84.33
48 with Daphniae 100 66.03

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): small (10/15 mL) glass containers
- Type of flow-through (e.g. peristaltic or proportional diluter): static test
- Renewal rate of test solution (frequency/flow rate): static test
- No. of organisms per vessel: 20 animals/concentration subdivided into batches of five animals each in small closed glass containers


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water used was that suggested by ISO 6341-1982
- Total organic carbon: not stated
- Particulate matter: not stated
- Metals: not stated
- Pesticides: not stated
- Chlorine: not stated
- Alkalinity: not stated
- Ca/Mg ratio: 4:1
- Conductivity: Not stated
- Culture medium different from test medium: stated to be similar
- Intervals of water quality measurement: 0 and 48 h


OTHER TEST CONDITIONS
- Adjustment of pH: not stated
- Photoperiod:8/16 h of dark/light daily
- Light intensity: not stated


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :


TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobilisation

Details on results:

None

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

EC50 and its statistical limits: by the trimmed Spearman-Karber Method (Hamilton,
M.A., R.C. Russo, and R.V. Thurston, 1977. Trimmed Spearman-Karber method for
estimating medium lethal concentrations in toxicity bioassays. Environ. Sci. Technol,
11(7): 714-719; correction 12(4): 27(1978).

Cumulative immobilisation

 

mg/L	24 h		48 h
	No. immobilised animals	% immobilisation	No. immobilised animals	% immobilisation
0	0	0	1	5
100	2	10	5	25

Validity criteria fulfilled:

yes

Conclusions:

Experimental data from an acute immobilization test in which Daphnia magna were treated with the test article DIMETHYLCARBONATE at the nominal
concentration of 100 mg/L are given in this report. The animals were treated and observed for 48 hours .
One animal was immobilized in the control group at 48 h observation time.
Group 1: 2 and 5 animals were immobilized at 24 and 48 hours respectively.
The ECso of DIMETHYLCARBONATE at 24 and at 48 hours were >100 mg/L, (83.30 and 74.16 mg/L. measured concentrations).

Description of key information

Experimental data from an acute immobilization test in which Daphnia magna were treated with the test article Dimethyl carbonate at the nominal concentration of 100 mg/L are given in this report. The animals were treated and observed for 48 hours . One animal was immobilized in the control group at 48 h observation time.
The 48h EC50 has been determined to be >100 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L