Registration Dossier
Registration Dossier
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EC number: 266-030-3 | CAS number: 65996-95-4 Substance obtained by acidulating phosphate rock with phosphoric acid. Normally characterized as containing 40% or more available phosphoric oxide (P2O5). Composed primarily of calcium phosphate.
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Based on a reliable study with triple superphosphate the EC50 for freshwater algae is >87.6 mg/L (> 100 mg/L nominal) and the NOEC is ≥87.6 mg/L (> 100 mg/L nominal).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 87.6 mg/L
- EC10 or NOEC for freshwater algae:
- 87.6 mg/L
Additional information
In a OECD 201 guideline study, Selenastrum capricornutum algae were exposed for 72 hrs to 6.25, 12.5, 25.0, 50.0, and 100 mg/L of triple superphosphate under static conditions. Measured concentrations (mean calculated) were 4.83, 10.3, 21.6, 42.6 and 87.6 mg/L (77 -88% of nominal). No adverse inhibitory toxic effects were observed. The substance produced a stimulation of algal growth (biomass) at all concentrations tested that ranged from 6 to 24% compared to the control biomass. Algal growth rate was inhibited or consistent with the growth rate of the control algae for the 4.83 and 21.6 mg/L concentrations. All other test concentrations demonstrated slight stimulation (2 to 5%) in the algal growth rate as compared to the control growth rate.
Therefore, EbC50 and ErC50 values for inhibition of algal growth could not be calculated. The NOEC was determined to be 87.6 mg/L (100 mg/L nominal concentration) based on the lack of significant inhibition or stimulation of algal growth (biomass and growth rate) as compared to the control.
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