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EC number: 229-347-8 | CAS number: 6484-52-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- The study was not carried out according to GLP and no analytical monitoring and no standard medium was used.
- Principles of method if other than guideline:
- As per Anderson, B.G., Andrews, T.F., Chandler, D.G., and Jahoda, W.J. 1948. The evaluation of aquatic invertebrates as assay organisms for the determination of toxicity of industrial wastes. Report from Ohio State University Research Foundation to the American Petroleum Institute, New York, 1948.
- Measurements up to 96h were done. - GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia Magna
- Source: cultured from a starting culture originally obtained from Put-In-Bay, Ohio, US. - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Details on test conditions:
- TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: University Lake water (ULW) filtered through glass wool
- Source/preparation of dilution water: Standard Reference Water (SRW) - Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 490 mg/L
- Remarks on result:
- other: University Lake water
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 490 mg/L
- Remarks on result:
- other: University Lake water
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 226 mg/L
- Remarks on result:
- other: University Lake water
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 39 mg/L
- Remarks on result:
- other: University Lake water
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 900 mg/L
- Remarks on result:
- other: Standard Reference Water
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- TLm (=EC50) at 48hours = 490 mg/L (300 mg NO3/L)
Reference
Description of key information
No reliable study with ammonium nitrate was available. A reliable study with potassium nitrate showed an 48-hr EC50 of 490 mg/L. The read-across rationale can be found in the target study record.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 490 mg/L
Additional information
No reliable study with ammonium nitrate was available. However, a daphnia study with potassium nitrate was performed. This study was performed according to Anderson, B.G., Andrews, T.F., Chandler, D.G., and Jahoda, W.J. 1948. The evaluation of aquatic invertebrates as assay organisms for the determination of toxicity of industrial wastes. Report from Ohio State University Research Foundation to the American Petroleum Institute, New York, 1948. Daphnia were exposed for 96 hrs, however the 48 -hr endpoint has been used here and was determined to be 490 mg/L. The study endpoint was expressed as the TLm (median Tolerance Limit). The median Tolerance Limit is the concentration of a toxic material at which 50% of the testorganisms (usually aquatic organisms) survive (=EC50).
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