Ammonium nitrate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

The study was not carried out according to GLP and no analytical monitoring and no standard medium was used.

Principles of method if other than guideline:

As per Anderson, B.G., Andrews, T.F., Chandler, D.G., and Jahoda, W.J. 1948. The evaluation of aquatic invertebrates as assay organisms for the determination of toxicity of industrial wastes. Report from Ohio State University Research Foundation to the American Petroleum Institute, New York, 1948.
- Measurements up to 96h were done.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia Magna
- Source: cultured from a starting culture originally obtained from Put-In-Bay, Ohio, US.

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: University Lake water (ULW) filtered through glass wool
- Source/preparation of dilution water: Standard Reference Water (SRW)

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

490 mg/L

Remarks on result:

other: University Lake water

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

490 mg/L

Remarks on result:

other: University Lake water

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

226 mg/L

Remarks on result:

other: University Lake water

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

39 mg/L

Remarks on result:

other: University Lake water

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

900 mg/L

Remarks on result:

other: Standard Reference Water

Validity criteria fulfilled:

not applicable

Conclusions:

TLm (=EC50) at 48hours = 490 mg/L (300 mg NO3/L)

Description of key information

No reliable study with ammonium nitrate was available. A reliable study with potassium nitrate showed an 48-hr EC50 of 490 mg/L. The read-across rationale can be found in the target study record.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

490 mg/L

Additional information

No reliable study with ammonium nitrate was available. However, a daphnia study with potassium nitrate was performed. This study was performed according to Anderson, B.G., Andrews, T.F., Chandler, D.G., and Jahoda, W.J. 1948. The evaluation of aquatic invertebrates as assay organisms for the determination of toxicity of industrial wastes. Report from Ohio State University Research Foundation to the American Petroleum Institute, New York, 1948. Daphnia were exposed for 96 hrs, however the 48 -hr endpoint has been used here and was determined to be 490 mg/L. The study endpoint was expressed as the TLm (median Tolerance Limit). The median Tolerance Limit is the concentration of a toxic material at which 50% of the testorganisms (usually aquatic organisms) survive (=EC50).