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EC number: 229-347-8 | CAS number: 6484-52-2
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: cytogenicity / chromosome aberration
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- Not a guideline study. The information given is limited to the above mentioned. The aberration frequency at 5.2 mg/kg bw is without biological meaning and may be a mistake in the table. It is not clear whether a metaphase-arresting agent was added before sacrifice.
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of the mutagenic activity of ammonium nitrate and dimethylamine salt of 2,4-dichlorophenoxyacetic acid
- Author:
- Nechkina, MA
- Year:
- 1 992
- Bibliographic source:
- Gigiena i sanitariia (2); p. 66-7
Materials and methods
- Principles of method if other than guideline:
- Only one sex used, sampling earlier than 1.5 normal cell cycle; no positive controls.
- GLP compliance:
- not specified
- Type of assay:
- chromosome aberration assay
Test material
- Reference substance name:
- Ammonium nitrate
- EC Number:
- 229-347-8
- EC Name:
- Ammonium nitrate
- Cas Number:
- 6484-52-2
- Molecular formula:
- H3N.HNO3
- IUPAC Name:
- ammonium nitrate
- Test material form:
- solid
- Remarks:
- granules or crystals
- Details on test material:
- No further details provided.
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- not specified
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- - Sex: male
- Age: sexually-mature
- Number: 6/dose
- Controls: 9 animals vehicle only
Administration / exposure
- Route of administration:
- oral: gavage
- Duration of treatment / exposure:
- 15 days
- Frequency of treatment:
- daily
- Post exposure period:
- Sampling times and number of samples: 6 h after last administration
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0 mg/kg bw (total dose)
- Dose / conc.:
- 5.2 mg/kg bw (total dose)
- Dose / conc.:
- 20.9 mg/kg bw (total dose)
- Dose / conc.:
- 104 mg/kg bw (total dose)
- Dose / conc.:
- 417 mg/kg bw (total dose)
- No. of animals per sex per dose:
- 6/dose
- Control animals:
- other: 9 animals vehicle only (vehicle = water)
Examinations
- Tissues and cell types examined:
- Chromosome preprations were prepared using the standard Ford method from bone marrow cells; 100 metaphases were examined per animal.
Results and discussion
Test results
- Key result
- Sex:
- male
- Genotoxicity:
- negative
- Remarks:
- No increase in aberrations was found in the treated groups relative to the negative control.
- Additional information on results:
- EFFECT ON MITOTIC INDEX OR PCE/NCE RATIO: not indicated
GENOTOXIC EFFECTS: No increase in aberrations was found in the treated groups relative to the negative control.
ABERRATION FREQUENCY:
- Number of metaphases with aberrations (gaps excluded): -0.5, 0.3, 0.7, 0.8 and 1.1 per 100 metaphases for 0, 5.2, 20.9, 104 and 417 mg/kg bw, respectively
Applicant's summary and conclusion
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