Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08 April 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
not specified
GLP compliance:
not specified
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- code number: FAT 93460/A
- Source and batch No.of test material: EN 037098A7
- Expiration date of the batch: November, 2003

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature

OTHER SPECIFICS:
- Purity: ca. 23 %
- Appearance: yellow, solid
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge
Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
3 h
pH:
7.6 - 7.8
Dissolved oxygen:
5.4 - 6.6 (O2 consumption in cm)
Nominal and measured concentrations:
25.6, 64, 160, 400 and 1000 mg/L (nominal concentrations)
Reference substance (positive control):
not specified
Key result
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
0.00, 0.00, 3.89, 0.00 and 1.62 % inhibition was recorded for concentrations 25.6, 64, 160, 400 and 1000 mg/L, respectively.
Results with reference substance (positive control):
The bacteria toxicity of the reference substance 3.5-Dichlorophenol (IC50 3h) was: 13 mg/L

Results:

Test substance (mg/L)

pH after 3h

O2 - consumption (in cm)

 Time (in cm)

mg O2 pro min.II

 % of control

 % inhibition

1000

7.6

5.6

5.3

0.85

98.38

1.62

400

7.8

6.6

5.9

0.89

104.15

0.00

160

7.8

6.4

6.2

0.83

96.11

3.89

64

7.8

6.1

5.6

0.87

101.42

0.00

25.6

7.8

5.4

4.7

0.92

106.97

0.00
Validity criteria fulfilled:
yes
Conclusions:
The IC50 (3 h) was determined to be >1000 mg/L.
Executive summary:

The toxicity of the test substance to bacteria was investigated in a study conducted according to OECD Guideline 209. The bacterial populations were exposed to test concentrations of 25.6, 64, 160, 400 and 1000 mg/L for 3 hours and observed for inhibition. 0.00, 0.00, 3.89, 0.00 and 1.62 % inhibition was recorded for concentrations 25.6, 64, 160, 400 and 1000 mg/L, respectively. Based on the findings of the study, the IC50 (3 h) was determined to be >1000 mg/L.

Description of key information

The toxicity of the test substance to bacteria was investigated in a study conducted according to OECD Guideline 209. The bacterial populations were exposed to test concentrations of 25.6, 64, 160, 400 and 1000 mg/L for 3 hours and observed for inhibition. 0.00, 0.00, 3.89, 0.00 and 1.62 % inhibition was recorded for concentrations 25.6, 64, 160, 400 and 1000 mg/L, respectively. Based on the findings of the study, the IC50 (3 h) was determined to be >1000 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L

Additional information