Calcium tartrate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 January 2011 - 31 January 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
Limit test: 100.2 mg/L

- Sampling method: The treatment solutions were prepared in reconstituted water.

- Sample storage conditions before analysis:
The tubes were maintained in the dark in a climatic chamber. The temperature was continuously controlled, using a calibrated thermo-recorder.
Range-finding test 20.0 to 20.5°C
Limit test 20.2 to 20.4°C

Vehicle:

no

Test organisms (species):

Daphnia sp.

Details on test organisms:

TEST ORGANISM
- Strain: D Magna
- Source: from a commercial breeder and regularly sub-cultured at the Phytosafe site
- Age at study initiation (mean and range, SD): adult females -> young daphnids produced within 24 hours were used for the tests
- Not fed

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

no

Test temperature:

20.2 to 20.4°C

pH:

7.7

Dissolved oxygen:

7.9-8.7 mg/L

Nominal and measured concentrations:

Range-finding test: 0.01 – 0.1 – 1.0 – 10.0 and 100.2 mg/L
Limit-test: 100.2 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: tubes
- Type (delete if not applicable): loosely capped
- No. of organisms per vessel: Five daphnids per unit.
- No. of vessels (replicates): Limit test: Four replicate units for the test item treatments, for the reference item treatments and for the control.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The M4 mineral medium was freshly reconstituted from analytical grade water using stock solutions of mineral components

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: in the dark

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No daphnid was immobile.

Results with reference substance (positive control):

EC50-24h: 1.32 mg/L

Validity criteria fulfilled:

yes

Conclusions:

Short-term toxicity to aquatic invertebrates of Calcium tartrate was determined according to OECD Guideline 202. As a result of this test 48 h NOEC ≥ 100 mg/L.

Executive summary:

Phytosafe Study Number:	10-27-076-ES
Study:	Daphnia sp., Acute immobilization test with Calcium tartrate (OECD 202, April 2004)
Test item:	Calcium tartrate Batch No.Camp 09.10
Nominal test concentrations:	Range-finding test:– 0.1 – 1.0 – 10.0 and 100.2 mg/L Limit test: 100.2 mg/L
Length of test:	48-hour exposure period

 

Test organisms	Daphnia magna
Source	Phytosafe s.a.r.l. 2,rue Marx Dormoy. 64000 PAU. France
Age	Less than 24 hours at test initiation
Control	Water
Toxic standard	Potassium dichromate (EC50-24 h between 0.6 and 1.7 mg/L)

 

Results	Range-finding test	Limit test
NOEC-24 h	≥ 100 mg/L	≥ 100 mg/L
NOEC-48 h	≥ 100 mg/L	≥ 100 mg/L
Recovery of the test item at the end of test		89.1 %

Description of key information

Short-term toxicity to aquatic invertebrates of Calcium tartrate was determined according to OECD Guideline 202. As a result of this test 48 h NOEC ≥ 100 mg/L.