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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
24 January 2011 - 31 January 2011
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
- Concentrations:
Limit test: 100.2 mg/L

- Sampling method: The treatment solutions were prepared in reconstituted water.

- Sample storage conditions before analysis:
The tubes were maintained in the dark in a climatic chamber. The temperature was continuously controlled, using a calibrated thermo-recorder.
Range-finding test 20.0 to 20.5°C
Limit test 20.2 to 20.4°C
Vehicle:
no
Test organisms (species):
Daphnia sp.
Details on test organisms:
TEST ORGANISM
- Strain: D Magna
- Source: from a commercial breeder and regularly sub-cultured at the Phytosafe site
- Age at study initiation (mean and range, SD): adult females -> young daphnids produced within 24 hours were used for the tests
- Not fed
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
no
Test temperature:
20.2 to 20.4°C
pH:
7.7
Dissolved oxygen:
7.9-8.7 mg/L
Nominal and measured concentrations:
Range-finding test: 0.01 – 0.1 – 1.0 – 10.0 and 100.2 mg/L
Limit-test: 100.2 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: tubes
- Type (delete if not applicable): loosely capped
- No. of organisms per vessel: Five daphnids per unit.
- No. of vessels (replicates): Limit test: Four replicate units for the test item treatments, for the reference item treatments and for the control.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The M4 mineral medium was freshly reconstituted from analytical grade water using stock solutions of mineral components

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: in the dark
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
No daphnid was immobile.
Results with reference substance (positive control):
EC50-24h: 1.32 mg/L
Validity criteria fulfilled:
yes
Conclusions:
Short-term toxicity to aquatic invertebrates of Calcium tartrate was determined according to OECD Guideline 202. As a result of this test 48 h NOEC ≥ 100 mg/L.
Executive summary:

Phytosafe Study Number:

10-27-076-ES

Study:

Daphnia sp., Acute immobilization test with Calcium tartrate (OECD 202, April 2004)

Test item:

Calcium tartrate

Batch No.Camp 09.10

Nominal test concentrations:

Range-finding test:– 0.1 – 1.0 – 10.0 and 100.2 mg/L

Limit test: 100.2 mg/L

Length of test:

48-hour exposure period

 

Test organisms

Daphnia magna

Source

Phytosafe s.a.r.l. 2,rue Marx Dormoy. 64000 PAU. France

Age

Less than 24 hours at test initiation

Control

Water

Toxic standard

Potassium dichromate (EC50-24 h between 0.6 and 1.7 mg/L)

 

Results

Range-finding test

Limit test

NOEC-24 h

≥ 100 mg/L

≥ 100 mg/L

NOEC-48 h

≥ 100 mg/L

≥ 100 mg/L

Recovery of the test item at the end of test

 

89.1 %

Description of key information

Short-term toxicity to aquatic invertebrates of Calcium tartrate was determined according to OECD Guideline 202. As a result of this test 48 h NOEC ≥ 100 mg/L.

Key value for chemical safety assessment

Additional information

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