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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study has been assessed for the use in a category approach. According to the methodology and to the extent of available details, the study has been judged as reliable with restrictions.

Data source

Reference
Reference Type:
publication
Title:
Total an d 1( +) Tartaric Acid lnhibited Rat Serum Acid Phosphatase
Author:
Z. LARON B. EPSTEIN HALBERSTADT
Year:
1960

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
1( +) Tartaric Acid
IUPAC Name:
1( +) Tartaric Acid
Test material form:
not specified
Radiolabelling:
no

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female

Administration / exposure

Route of administration:
other: not specified
Vehicle:
not specified
Doses / concentrations
Remarks:
Doses / Concentrations:
The purpose of the present investigation was to determine the activities of the total and L( +) tartaric acid inhibited acid phosphatase in the serum of rats of both sexes.
No. of animals per sex per dose / concentration:
I Group Male 40-50 gr 12 rats
II Group Male 140-180 gr 19 rats
III Group Female 40-50 gr 8 rats
IV Group Female 120-160gr 10 rats
Control animals:
not specified

Results and discussion

Preliminary studies:
no data

Metabolite characterisation studies

Metabolites identified:
no

Any other information on results incl. tables

It is evident that the activity of both serum total and L(+) tartaric acid inhibited acid phosphatase is much higher in the rat than in the human serum in which the mean activity is 2 ·5 KingArmstrong units in adults and 5 King-Armstrong units in children.

Serum total and L(+)tartaric acid inhibited acid phosphatase in rats

Group        Sex       Wheight       #rats       Serum total acid phosphatase*      Serum L(+)tartaric acid inhibited acid phospatase*

I              male       40 -50 gr       12              18.5 +/-2.98                                   6.79 +/-2.3

II             male       140 -180 gr    19              17.3 +/-4.92                                   8.68 +/-3.4

III          female     40 -50 gr        8                17.3 +/-2.1                                     7.3 +/-2.93

IV          female     120 -160 gr   10               14.9 +/-3.17                                   6.7 +/-1.26

*king-Armstrong units mean ± S.D.

White rats were anresthetized with 'Nembutal'. Blood was drawn from the abdominal aorta, twlce centrifuged and the enzyme activities determined immediately thereafter according to the methods of King

               

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): bioaccumulation potential cannot be judged based on study results
It seems that younger rats do not bave statistically higher serum acid phosphatase activity than older ones such as in humans , but without serial determinations at many ages starting in very young animala this question cannot be definitely answered.