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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from Authoritive Database

Data source

Reference
Reference Type:
other: Authoritive Database
Title:
Skin irritation for CAS NO: 60-00-4
Author:
IFA (GESTIS)
Year:
2017
Bibliographic source:
GESTIS SUBSTANCE Database (information system in hazardous substance of the Berufsgenossenscheftn)

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
A Skin irritation study was conducted in rabbits to assess the irritation potential of Ethylenediaminetetraacetic acid (EDTA)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetic acid
Cas Number:
60-00-4
Molecular formula:
C10H16N2O8
IUPAC Name:
2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetic acid
Test material form:
solid
Details on test material:
2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetic acid /60-00-4/200-449-4
- Name of test material (as cited in study report): Ethylenediaminetetraacetic acid (EDTA)
- Molecular formula: C10H16N2O8
- Molecular weight: 292.3 g/mol
- Substance type: Organic
- Physical state: Solid
-Smiles: N(CCN(CC(O)=O)CC(O)=O)(CC(O)=O)CC(O)=O
-InChI: 1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1 6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
Specific details on test material used for the study:
2,2',2'',2'''-(ethane-1,2-diyldinitrilo)tetraacetic acid /60-00-4/200-449-4
- Name of test material (as cited in study report): Ethylenediaminetetraacetic acid (EDTA)
- Molecular formula: C10H16N2O8
- Molecular weight: 292.3 g/mol
- Substance type: Organic
- Physical state: Solid
-Smiles: N(CCN(CC(O)=O)CC(O)=O)(CC(O)=O)CC(O)=O
-InChI: 1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1 6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

Test animals

Species:
rabbit
Strain:
not specified
Details on test animals or test system and environmental conditions:
No Data Available

Test system

Type of coverage:
not specified
Preparation of test site:
not specified
Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
50% aqueous solution
Duration of treatment / exposure:
No Data Available
Observation period:
20 hours
Number of animals:
No Data Available

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 20 hours
Score:
0
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
No skin irritating effects were observed.

Applicant's summary and conclusion

Interpretation of results:
other: not irritating
Conclusions:
No known signs of irritation were observed after 20 days. Hence, Ethylenediaminetetraacetic acid (CAS No: -60-00-4) wasconsidered to be non irritating to the rabbits skin.
Executive summary:

Skin irritation study was carried out on rabbits to assess the irritation potential of test chemical Ethylenediaminetetraacetic acid (CAS No: -60-00-4).

The skins of rabbits were exposed to the test chemical at concentration of 50% in aqueous solution and skin reactions were observed up to 20 days.

No known signs of irritation were observed after 20 days. Hence, Ethylenediaminetetraacetic acid (CAS No: -60-00-4) wasconsidered to be non irritating to the rabbits skin.