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Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
genetic toxicity in vitro, other
Remarks:
gene mutation study in yeast
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reviewed publication

Data source

Reference
Reference Type:
publication
Title:
Testing of chemicals for mutagenic activity with Schizosaccharomyces pombe- A report of the U.S. Environmental Protection Agency Gene-Tox Program
Author:
N. Loprieno, R. Barale , E.S. Von Halle and R.C. yon Borstel
Year:
1983
Bibliographic source:
Mutation Research, 115 (1983) 215-223

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Refer below principle
Principles of method if other than guideline:
Reverse mutation was studied for the 2-Bromoethaneamine hydrobromide using yeast Schizosaccharomyces pombe
GLP compliance:
not specified
Type of assay:
other: Schizosaccharomyces pombe gene mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-bromoethylammonium bromide
EC Number:
219-924-2
EC Name:
2-bromoethylammonium bromide
Cas Number:
2576-47-8
Molecular formula:
C2H6BrN.BrH
IUPAC Name:
2-bromoethanaminium bromide
Test material form:
solid
Details on test material:
SMILES:BrCCN{+}.Br{-}
Specific details on test material used for the study:
- Name of test material: 2-Bromoethaneamine hydrobromide
- Molecular formula: C2H6BrNBrH
- Molecular weight: 204.892 g/mol
- Substance type: Organic
- Physical state: No data
- Purity: No data
- Impurities (identity and concentrations): No data

Method

Target gene:
his52, his7, met4, ade6 and ade7
Species / strain
Species / strain / cell type:
yeast, other: Schizosaccharomyces pombe strain Arg 1
Details on mammalian cell type (if applicable):
Not applicable
Additional strain / cell type characteristics:
not specified
Cytokinesis block (if used):
No data
Metabolic activation:
without
Metabolic activation system:
Exogeneous metabolic activation system- S9 and S100
Test concentrations with justification for top dose:
No data
Vehicle / solvent:
No data
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified
Details on test system and experimental conditions:
No data
Rationale for test conditions:
No data
Evaluation criteria:
The yeast cells were observed for reversion of mutation
Statistics:
No data

Results and discussion

Test results
Species / strain:
yeast, other: Schizosaccharomyces pombe strain Arg 1
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
No data

Any other information on results incl. tables

Table: Chemicals Tested for Mutagenicity on Stationary Phase Cells of Schizosaccharomyces pombe

Chemical

Strain

Metabolic activation

Genetic end point

Genetic activity

2-Bromoethaneamine hydrobromide

Arg 1

-

Reverse mutation

+

 

Applicant's summary and conclusion

Conclusions:
2-Bromoethaneamine hydrobromide induced reversion of mutation in yeast Schizosaccharomyces pombe and hence the chemical is likely to be mutagenic to yeast cells.
Executive summary:

Reverse mutation was studied for the 2-Bromoethaneamine hydrobromide using yeast Schizosaccharomyces pombe strain Arg 1. The study was performed without exogeneous metabolic activation system at either of the his52, his7, met4, ade6 and ade7 locus. All these systems allow the evaluation of back mutation induction at the original mutant site. 2-Bromoethaneamine hydrobromide induced reversion of mutation in yeast Schizosaccharomyces pombe without exogeneous metabolic activation system and hence the chemical is likely to be mutagenic to yeast cells.