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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
None
Qualifier:
according to guideline
Guideline:
other: CIBA Method A-11
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
In principle the methods described in OECD Guideline 203 were used.
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
None
Vehicle:
yes
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen

Golden orfe: (Leuciscus idus)
Replicates: 8 per test concentration
Age: about 12 months
Size: 3-4 cm
Weight: 1.5-2.5 g


Zebrafish: (Danio rerio)
Replicates: 10 per test concentration
Age: about 4-6 months
Size: about 3 cm
Weight: 0.25-0.35 g
Supplier: West Aquarium GmbH, D-3422 Bad Lauterberg
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
None
Test temperature:
Zebra fish: 23 °C
pH:
7.8
Dissolved oxygen:
None
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
300 and 1000 mg/L
Details on test conditions:
Water:
15 liters for trout and orfe or 3 liters for zebrafish (active coal filtered). Water used according to ISO guidelines.
Basins:
Full-glass basins, 17 liters basis (36x23x26 cm) tempered (cold-room); 5 liter basins (15x20x20 cm) tempered
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
300 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
No mortality occurred at 300 mg/L, while 2 out of 10 fish died at 1000 mg/L concentration.
Results with reference substance (positive control):
None
Reported statistics and error estimates:
None
Sublethal observations / clinical signs:

None

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 of FAT 40032/D in this fish acute toxicity test was determined to be >1000 mg/L.
Executive summary:

The acute fish toxicity test was performed according to a methodology similar to OECD Guideline 203. Zebrafish: (Danio rerio) were used in the experiment setup. Fish were tested at these different following concentrations: 320 and 1000 mg/L. No mortality occurred at 300 mg/L, while 2 out of 10 fish died at 1000 mg/L concentration.

In conclusion the results obtained were.

LC0 = 300 mg/L

LC50 >1000 mg/L

LC100 >1000 mg/L.

Description of key information

The LC50 of FAT 40032/D in the fish acute toxicity test was determined to be greater than 1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1 000 mg/L

Additional information

Acute toxicity of Reactive Brown 011 to fish was determined in four available studies. The outcome of all these studies, indicated the LC50 to be >1000 mg/L.

In the key study, Zebrafish: (Danio rerio) were exposed to FAT 40032/D at 300 and 1000 mg/L for 96 hours. No mortality occurred at 300 mg/L while 2 out of 10 fish were found dead at 1000 mg/L concentration.

In conclusion the results obtained were:

LC0 = 300 mg/L

LC50 >1000 mg/L

LC100 >1000 mg/L.

Several other studies are available, results of which are presented below:

FAT 40032/A:

TL0 = 1000 mg/L

TL50 = >1000 mg/L

TL100 = >1000 mg/L

FAT 40032/B: (96 h)

TL0 = 1000 mg/L

TL50 >1000 mg/L

TL100 >1000 mg/L

FAT 40032/C: (96 h)

TL0 = 300 mg/L

TL50 >1000 mg/L

TL100 >1000 mg/L