Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
None
Qualifier:
according to guideline
Guideline:
other: CIBA Method A-11
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
In principle the methods described in OECD Guideline 203 were used.
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
None
Vehicle:
yes
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen

Golden orfe: (Leuciscus idus)
Replicates: 8 per test concentration
Age: about 12 months
Size: 3-4 cm
Weight: 1.5-2.5 g

Zebrafish: (Danio rerio)
Replicates: 10 per test concentration
Age: about 4-6 months
Size: about 3 cm
Weight: 0.25-0.35 g
Supplier: West Aquarium GmbH, D-3422 Bad Lauterberg
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
None
Test temperature:
Zebrafish: 23 °C
pH:
7.8
Dissolved oxygen:
None
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
300 and 1000 mg/L
Details on test conditions:
Water:
15 liters for trout and orfe or 3 liters for zebrafish (active coal filtered). Water used according to ISO guidelines.
Basins:
Full-glass basins, 17 liters basis (36x23x26 cm) tempered (cold-room); 5 liter basins (15x20x20 cm) tempered
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
300 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
No mortality occurred at 300 mg/L, while 2 out of 10 fish died at 1000 mg/L concentration.
Results with reference substance (positive control):
None
Reported statistics and error estimates:
None
Sublethal observations / clinical signs:

None

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 of FAT 40032/D in this fish acute toxicity test was determined to be >1000 mg/L.
Executive summary:

The acute fish toxicity test was performed according to a methodology similar to OECD Guideline 203. Zebrafish: (Danio rerio) were used in the experiment setup. Fish were tested at these different following concentrations: 320 and 1000 mg/L. No mortality occurred at 300 mg/L, while 2 out of 10 fish died at 1000 mg/L concentration.

In conclusion the results obtained were.

LC0 = 300 mg/L

LC50 >1000 mg/L

LC100 >1000 mg/L.

Description of key information

The LC50 of FAT 40032/D in the fish acute toxicity test was determined to be greater than 1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1 000 mg/L

Additional information

Acute toxicity of Reactive Brown 011 to fish was determined in four available studies. The outcome of all these studies, indicated the LC50 to be >1000 mg/L.

In the key study, Zebrafish: (Danio rerio) were exposed to FAT 40032/D at 300 and 1000 mg/L for 96 hours. No mortality occurred at 300 mg/L while 2 out of 10 fish were found dead at 1000 mg/L concentration.

In conclusion the results obtained were:

LC0 = 300 mg/L

LC50 >1000 mg/L

LC100 >1000 mg/L.

Several other studies are available, results of which are presented below:

FAT 40032/A:

TL0 = 1000 mg/L

TL50 = >1000 mg/L

TL100 = >1000 mg/L

FAT 40032/B: (96 h)

TL0 = 1000 mg/L

TL50 >1000 mg/L

TL100 >1000 mg/L

FAT 40032/C: (96 h)

TL0 = 300 mg/L

TL50 >1000 mg/L

TL100 >1000 mg/L