Carbamic acid,N,N'-[1,3-phenylenebis[methyleneiminocarbonylimino(methyl-3,1-phenylene)]]bis-di-C11-14-isoalkylesters, C13-rich

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Samples were taken (at least in duplicate) from each concentration during the Limit Test at 0, 24, 48, 72 and 96 h. On each occasion, one sample collected was filtrated by 0.45 run millipore membrane (discarduy: the initial 10 mL fdtrates). then analysed by TOC; the remaining samples were retained in case further analysis would be required.

Vehicle:

no

Test organisms (species):

other: Brachydanio rerio

Details on test organisms:

The test species of Zebra fish. Brachydanio rerio (Batch No.: F20120915). were obtained as fry from a commercial fish supplier of Guangzhou Hongfa Aquatic Animals Culture Company in China. Feeding fry were held for 18 days in holding tanks supplied with a continuous flow of aerated water before being used for testing. Fish to be used in the test was held for 7 days in water of the quality and temperature to be used in the Test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

119 mg (CaCO3)/L - 125 mg (CaCO3)/L

Test temperature:

22.5°C to 23.3°C

pH:

7.45 - 7.61

Dissolved oxygen:

87%- 91%

Nominal and measured concentrations:

nominal 100 mg/L
measured concentration 0.78 mg/L

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: TOC

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: TOC

Validity criteria fulfilled:

yes

Conclusions:

The results showed that under valid static test conditions, the 96 h-LC50 Addukt TI80-MXDA to Zebra fish is greater that the nominal concentration of 100 mg/L WAFs (measured concentration 0.78 mg/L), while the maximum concentration causing no mortality (96 h-LC0) is equal to the nominal concentration of 100 mg/L WAFs (measured concentration 0.78 mg/L).
96 h-LC50 > 100 mg/L WAFs (measured concentration 0.77 mg/L);

96 h-LC0 = 100 mg/L WAFs (measured concentration 0.77 mg/L).

Executive summary:

Under static conditions, the acute toxicity of test item (Addukt TI80-MXDA) to Zebra fish (Brachydanio rerio) was conducted according to: “The guidelines for the testing of chemicals”, SEPA (HJ/T 153-2004); and with reference to Procedure 203 of the “Guidelines for Testing of Chemicals” of the OECD: “Fish, Acute Toxicity Test” (1992).

 

A range-finding test and then a Limit Test were performed respectively. Nominal concentrations of 1.0, 10 and 100 mg/L were used in the range-finding-test, while nominal concentrations of 100 mg/L WAFs was used in the Limit Test. Water samples taken from the control and the treatments in the Limit Test were analysed. The mean of the measured concentrations was 0.78 mg/L. The analytical results showed that the concentration of the test item is consistent in the test medium throughout the 96-hour test period (deviation within 20%). Thus a static procedure was reasonable.

 

In the range-finding test, 5 fishes per treatment and no replicates were used. And 10 fishes per treatment of no replicates were used in the Limit Test. The test fishes were exposed for 96 hours to the test solution.

 

During the whole test period, the pH values of the control mediums and test mediums were between 7.45 and 7.61, the Dissolved Oxygen (DO) values varied from 87% to 91% of the air saturation and the test temperature, and the total hardness was in the range of 119 mg (CaCO₃)/L to 125 mg (CaCO₃)/L. During the test, the temperature of the test mediums were maintained in the range of 22.5°C to 23.3°C, and all fishes in the control group were normal. With the same conditions, K₂Cr₂O₇was used as the positive control substance, and the resulting 24 h-LC₅₀was 262 mg/L. So the study met the acceptability criteria prescribed by the protocol (pH 6.0 ~ 8.5; dissolved oxygen concentration: >60% of the air saturation value; total hardness: 10 ~ 250 mg (CaCO₃)/L; temperature: (23±2) °C; 24 h- LC₅₀of K₂Cr₂O₇inthe range of 200 to 400 mg/L). Therefore the test was considered valid.

 

During the whole test period, all the fish in the control groups and the treated groups were alive and appeared normal. The results showed that under valid static test conditions, the 96 h- LC₅₀of Addukt TI80-MXDA to Zebra fish is greater than the nominal concentration of 100 mg/L WAFs (measured concentration 0.78 mg/L), while the maximum concentration causing no mortality (96 h-LC₀) is equal to the nominal concentration of 100 mg/L WAFs (measured concentration 0.78 mg/L).

 

96 h-LC₅₀> 100 mg/L WAFs (measured concentration 0.77 mg/L);

 

96 h-LC₀= 100 mg/L WAFs (measured concentration 0.77 mg/L).

Description of key information

96 h-LC50 Addukt TI80-MXDA to Zebra fish is greater that the nominal concentration of 100 mg/L WAFs (measured concentration 0.78 mg/L), while the maximum concentration causing no mortality (96 h-LC0) is equal to the nominal concentration of 100 mg/L WAFs (measured concentration 0.78 mg/L)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L