2-Naphthalenol, 1-[[2-methyl-4-[(2-methylphenyl)azo]phenyl]azo]-, ar-styrenated

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical :1. Short term toxicity test was performed for test material according t OECD guideline2.To evaluate short term toxicity of test material on fish

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): 2-Naphthalenol, 1-[[2-methyl-4-[(2-methylphenyl) azo] phenyl] azo]-, ar-styrenated- IUPAC name: 2-Naphthalenol, 1-[[2-methyl-4-[(2-methylphenyl) azo] phenyl] azo]-, ar-styrenated- Molecular formula: C40H36N4O1 - Molecular wt. : 588.76 g/mole- smiles : Cc1ccccc1N=Nc2ccc(c(C)c2)N=Nc3c4ccc(C(C)c5ccccc5)cc4cc(C(C)c6ccccc6)c3O- Substance type: Organic- Physical state: red liquid

Analytical monitoring:

not specified

Details on test solutions:

1.The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with 1 hr.continuous stirring for achieving test concentrations of 1g/L. Hence, concentrations are 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L ,respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM- Common name:ZEBRA FISH- Source:UNIQUE AQUARIUM, NAGPUR- Length at study initiation (length definition, mean, range and SD):average 1.94 cm- Weight at study initiation (mean and range, SD):0.1494 g (average)ACCLIMATION- Acclimation period:7 days- Type and amount of food during acclimation:Standard brand feed- Feeding frequency during acclimation:Daily once- Health during acclimation (any mortality observed):During last 48 hours = 0FEEDING DURING TEST- Food type:Standard brand feed- Frequency:Withdrawal of food 1 day before and during exposure

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

1) 148.5 mg of CaCO3

Test temperature:

1) 23 °C

pH:

1) 7.12

Dissolved oxygen:

1) 7.8 mg/l

Nominal and measured concentrations:

1) 0.3125mg/L, 0.625mg/L,1.25mg/L, 2.5mg/L, 5mg/L

Details on test conditions:

TEST ORGANISM- Common name:ZEBRA FISH- Source:UNIQUE AQUARIUM, NAGPUR- Length at study initiation (length definition, mean, range and SD):average 1.94 cm- Weight at study initiation (mean and range, SD):0.1494 g (average)ACCLIMATION- Acclimation period:7 days- Type and amount of food during acclimation:Standard brand feed- Feeding frequency during acclimation:Daily once- Health during acclimation (any mortality observed):During last 48 hours = 0FEEDING DURING TEST- Food type:Standard brand feed- Frequency:Withdrawal of food 1 day before and during exposure

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.312 - <= 0.625 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical -Naphthalenol, 1-[[2-methyl-4-[(2-methylphenyl) azo] phenyl] azo]-, ar-styrenated is not likely to be toxic to fish in the concentration range of 0.3125 to 0.60 mg/l

Executive summary:

Data available for the functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Naphthalenol, 1-[[2-methyl-4-[(2-methylphenyl) azo] phenyl] azo]-, ar-styrenated The studies are as mentioned below:

1.Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical . The test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with 1 hr.continuous stirring for achieving test concentrations of 1g/L. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. The lethal concentrations LC50 was determine to be >0.3125 mg/L but <0.625 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L.

2.Short term toxicity test for fish was performed to evaluate toxicity of test material on fish .The effect concentration of test material on fish was observed to be 0.60 mg/l after 96 h.

Thus, based on the above summarised studies, Naphthalenol, 1-[[2-methyl-4-[(2-methylphenyl) azo] phenyl] azo]-, ar-styrenated

and it’s functionally similar read across substance, it can be concluded that effect concetration of test substance is in the range of 0.3125 to 0.60 mg/l Thus, comparing this value with the criteria of CLP regulation can be classified as Aquatic chronic 1 for short term toxicity for fish .

Description of key information

Short term toxicity to fish:

Data available for the functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemicalNaphthalenol, 1-[[2-methyl-4-[(2-methylphenyl) azo] phenyl] azo]-, ar-styrenated The studies are as mentioned below:

1.Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical . The test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with 1 hr.continuous stirring for achieving test concentrations of 1g/L. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. The lethal concentrations LC50 was determine to be >0.3125 mg/L but <0.625 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L.

2.Short term toxicity test for fish was performed to evaluate toxicity of test material on fish .The effect concentration of test material on fish was observed to be 0.60 mg/l after 96 h.

Thus, based on the above summarised studies, Naphthalenol, 1-[[2-methyl-4-[(2-methylphenyl) azo] phenyl] azo]-, ar-styrenated

and it’s functionally similar read across substance, it can be concluded that effect concetration of test substance is in the range of 0.3125 to 0.60 mg/l Thus, comparing this value with the criteria of CLP regulationcan be classified as Aquatic chronic 1 for short term toxicity for fish .

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.312 mg/L

Additional information

Short term toxicity to fish:

Data available for the functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemicalNaphthalenol, 1-[[2-methyl-4-[(2-methylphenyl) azo] phenyl] azo]-, ar-styrenated The studies are as mentioned below:

1.Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical . The test solution was prepared by dissolving 100 mg of the test substance in 100 ml of potable water (passed through reverse osmosis system) with 1 hr.continuous stirring for achieving test concentrations of 1g/L. The nominal concentration selected for the experiment were 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/Land test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. The lethal concentrations LC50 was determine to be >0.3125 mg/L but <0.625 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 0.3125 mg/L LC50 (96 hours) Experimental = >0.3125 mg/L but <0.625 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 0.625 mg/L.

2.Short term toxicity test for fish was performed to evaluate toxicity of test material on fish .The effect concentration of test material on fish was observed to be 0.60 mg/l after 96 h.

Thus, based on the above summarised studies, Naphthalenol, 1-[[2-methyl-4-[(2-methylphenyl) azo] phenyl] azo]-, ar-styrenated

and it’s functionally similar read across substance, it can be concluded that effect concetration of test substance is in the range of 0.3125 to 0.60 mg/l Thus, comparing this value with the criteria of CLP regulationcan be classified as Aquatic chronic 1 for short term toxicity for fish .