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EC number: 283-640-5 | CAS number: 84696-21-9 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Hydrocotyle asiatica, Umbelliferae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 17 july 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Version / remarks:
- April 1996
- Deviations:
- no
- Principles of method if other than guideline:
- Description of the test procedure
Pre-Experiment
A preliminary test (static test) was performed at the saturation concentration (equivalent to 100 mg/L nominal concentration).
The test solution used in the preliminary test was prepared by mechanical dispersion. An amount of 0.2 g test item was dissolved in 2000 mL test medium (ISO medium) using approx. 10 min ultrasonic dispersion. This solution was shaken for about 1 hour and thereafter the non-dissolved test material was removed by filtration through a paper filter in order to give the saturated test solution (equivalent to 100 mg/L nominal concentration). The test solution was freshly prepared in the testing laboratory just before introduction of the test animals (start of the experiment).
Three fish were exposed to the test item treated and control group for 96 hours. No replicates were used. Both test vessels contained two litre test medium.
No mortality or any sub-lethal effects were noted in the treatment group or in the control group during the 96-h exposure period.
Test item concentrations in the Definitive Test
No test item effects were observed during the preliminary test. Therefore, a limit test was carried out with one treatment group at the saturation concentration (equivalent to 100 mg/L nominal concentration) and a concurrent control.
Performance of the Test
Body Weight Measurement and Introduction of Fish
At the start of the test (on day 0) 10 fish were introduced into each aquarium, each containing 5 litre testing solution. Before introduction of test animals, the body weight of fish was measured and recorded: first the weight of a vessel filled with water was determined. Then the 10 fish were selected randomly and placed into this vessel. The weight of the group of fish was registered and the average body weight calculated. On the basis of the above, the loading of the test-aquariums was calculated and were 0.475 g/L in the control and 0.458 g/L in the treatment group.
Observations
The fish were observed at approximately 3, 6, 24, 48, 72 and 96 hours after start of the test for signs of intoxication and mortality. Measurements of pH, dissolved oxygen and temperature were carried out daily (as described in section 5.5).
Body Length Measurement
The body length of each fish were measured at the end of the test in order to check their compliance with the size range recommended for the species by the test guideline (OECD No. 203). The measured values were within the range of 2.05-2.75 cm.
Analysis of Test Item Concentrations
On request of the Sponsor no determination of the test item concentration was performed during the study. - GLP compliance:
- yes (incl. QA statement)
- Vehicle:
- yes
- Remarks:
- water
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- The reconstituted water (ISO medium) has an approximate theoretical total hardness of 249 mg/L (as CaCO3).
- Test temperature:
- The aquariums were kept in a temperature-controlled tray. The measured temperatures were within a range of 22.8 – 23.6 °C in the test aquariums (the maximum deviation did not exceed ± 1°C).
- pH:
- The test was carried out without adjustment of pH. The measured pH values were in the range of 7.48 – 7.74 during the test.
- Dissolved oxygen:
- Oxygen Concentration: The dissolved oxygen concentration was in the range of 83.0 – 96.6 % of the air saturation value at the temperature used. Test solutions were not aerated during the test.
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- No determination of the test item concentration was performed during the study; therefore the biological results are based on the nominal concentration.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this 96-hour static acute toxicity test on Zebrafish (Brachydanio rerio) the test item CENTELLA ASIATICA had no toxic effect on fish up to the solubility limit of the test item in the test medium (equivalent to 100 mg/L nominal concentration). The 96-h NOEC was determined to be 100 mg/L.
Reference
Description of key information
In a 96-hour static acute toxicity test on Zebrafish (Brachydanio rerio) the test item CENTELLA ASIATICA had no toxic effect on fish up to the solubility limit of the test item in the test medium (equivalent to 100 mg/L nominal concentration). The 96-h NOEC was determined to be 100 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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