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EC number: 250-005-9 | CAS number: 30030-25-2
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 969
- Report date:
- 1969
Materials and methods
Test guideline
- Version / remarks:
- No OECD test guideline for skin sensitisation available when test was performed.
- Principles of method if other than guideline:
- not specified
- GLP compliance:
- no
- Remarks:
- GLP was not yet introduced at the time when the study was conducted.
- Type of study:
- not specified
- Justification for non-LLNA method:
- A skin sensitisation study was conducted on 6 guinea pigs. The test protocol which has not been specified in more detail in the study report is supposed to comply with one of various available guinea pig skin sensitization test protocols that measure cutaneous erythema and edema in suitably sensitized and challenged animals. At the time when the study was performed the ear flank test was a routine method. In this test, solutions of suspected sensitizing substance were applied over three days to the ears of guinea-pigs, and the flanks were challenged one week later with a range of concentrations of suspected sensitizing substance. The erythematous reaction produced 24 hours after challenge was rated and compared with that in unsensitized controls. Another non-adjuvant assay established at that time is the Buehler test.
Test material
- Reference substance name:
- (chloromethyl)vinylbenzene
- EC Number:
- 250-005-9
- EC Name:
- (chloromethyl)vinylbenzene
- Cas Number:
- 30030-25-2
- Molecular formula:
- C9H9Cl
- IUPAC Name:
- 1-(chloromethyl)-3-ethenylbenzene; 1-(chloromethyl)-4-ethenylbenzene
- Test material form:
- liquid
- Details on test material:
- The test material was a product of the direct chlorination of a 60/40 weight percent mixture of meta/para vinyl toluene. The actual ratio of the isomers was unknown.
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- not specified
- Sex:
- not specified
Study design: in vivo (non-LLNA)
- No. of animals per dose:
- 6
- Positive control substance(s):
- not specified
Results and discussion
In vivo (non-LLNA)
Resultsopen allclose all
- Key result
- Reading:
- other: positive (observed sensitisation response)
- Group:
- test chemical
- Dose level:
- not specified
- No. with + reactions:
- 4
- Total no. in group:
- 6
- Reading:
- other:
- Group:
- negative control
- Remarks on result:
- not determinable because of methodological limitations
- Reading:
- other:
- Group:
- positive control
- Remarks on result:
- not measured/tested
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (skin sensitising) based on GHS criteria
- Conclusions:
- Skin sensitisation studies conducted on guinea pigs resulted in a sensitivity response in 4 out of 6 of the surviving animals.
- Executive summary:
Skin sensitisation studies conducted on guinea pigs resulted in a sensitivity response in 4 out of 6 of the surviving animals.
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