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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
publication
Title:
Metabolic and Histologic Effects of Sodium PyruvateTreatment in the Rat after Cortical Contusion Injury
Author:
Masamichi Fukushima, Stefan M. Lee, Nobuhiro Moro, David A. Hovda, and Richard L. Sutton
Year:
2009
Bibliographic source:
JOURNAL OF NEUROTRAUMA 26:1095โ€“1110 (July 2009)ยช Mary Ann Liebert, Inc.
DOI: 10.1089=neu.2008.0771

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The publication provides suffient details on the study.
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium pyruvate
EC Number:
204-024-4
EC Name:
Sodium pyruvate
Cas Number:
113-24-6
Molecular formula:
C3H4O3.Na
IUPAC Name:
sodium 2-oxopropanoate
Test material form:
solid
Specific details on test material used for the study:
he SP (P2256, Sigma, St. Louis, MO) was dissolved in sterile water (500 mg=mL, 4.5 M, pH 7.4) immediately prior to scheduled injections.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male

Administration / exposure

Type of coverage:
other: intraperitoneal
Vehicle:
water
Details on dermal exposure:
Animals received an injection of sodium pyruvate (500 or 1000 mr/kg bw).
Doses:
500 mg/kg bw
1000 mg/kg bw
3x 1000 mg/kg bw hourly
No. of animals per sex per dose:
at least 5 per dose

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
> 3 000 mg/kg bw
Mortality:
not observed

Applicant's summary and conclusion

Conclusions:
Even with three hourly intraperitoneal injections of sodium pyruvate no deaths occured.
The uptake during dermal exposure with the same amount of substance would be lower. As a worst-case assumption it can be stated that the LC50, dermal is >= 3000 mg/kg bw.