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EC number: 203-305-9 | CAS number: 105-53-3
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- - Type of sludge: activated sludge, predominantly domestic
- Source: Sewage plant Marl-Ost
- Sampling site: activated sludge basin- Preparation of inoculum: Centrifugation 10 min at 1100 x g, the supernatant is discarded and the sludge resuspended with mineral medium, further centrifugation for 10 min at 1100 x g Resuspension of the activated sludge (2.79 g/l dry mass of activated sludge)
- Initial cell concentration: 27.9 mg/l - Duration of test (contact time):
- 28 d
- Initial conc.:
- 10.8 mg/L
- Based on:
- DOC
- Details on study design:
- - Composition of stock nutrient solutions:
a) 8.5 g/l KH2PO4
21.75 g/l K2HPO4
33.3 g/l Na2HPO4 * 2 H2O
20.0 g/l (NH4)Cl
b) 22.5 g/l MgSO4 * 7 H2O
c) 27.5 g/l CaCl2
d) 0.25 g/l FeCl3 * 6 H2O
- Additional substrate: No
- Test temperature: 21.8 - 22.1 °C
- Aeration of dilution water: no
- Concentration of suspended solids: 27.9 mg/l
- Addition of Stock nutrient solutions: a): 20 ml, b) - d):
2ml each.
CONTROLS: 1 Flask without test substance, but with inoculum,
REFERENCE SUBSTANCE: 2 Flasks with Benzoic acid, sodium salt,
10.64 mg DOC/l and inoculum. - Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 0 d
- Remarks on result:
- other: Flasks with test substance
- Parameter:
- % degradation (DOC removal)
- Value:
- 90
- Sampling time:
- 7 d
- Remarks on result:
- other: Flasks with test substance
- Parameter:
- % degradation (DOC removal)
- Value:
- 95
- Sampling time:
- 14 d
- Remarks on result:
- other: Flasks with test substance
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 21 d
- Remarks on result:
- other: Flasks with test substance
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 28 d
- Remarks on result:
- other: Flasks with test substance
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Due to the study results the substance can be stated as readily biodegradable meeting the 10-day-window.
Reference
Description of key information
Due to the study results the substance can be stated as readily biodegradable meeting the 10-day-window.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
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