Diethyl malonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

CaCl2 x 2 H2O: 294 mg/l, MgSO4 x 7 H2O: 123 mg/l

Test temperature:

20 +- 2°C

pH:

7.0 to 7.5

Dissolved oxygen:

6.6 to 7.8 mg/l

Salinity:

- KCl: 5.5 mg/l
- Ca/Mg ratio: 4 : 1
- Na/K ratio: 10 : 1

Nominal and measured concentrations:

100, 140, 200, 280, 400, 560, 800 mg

Details on test conditions:

TEST ORGANISMS
- Strain: Daphnia magna Straus Clone 5
- Source/supplier: Hüls AG
- Breeding method: Breeding method according to Elendt (1990) in M4-medium in 1l beakers, water exchange every 2 to 3 days.
- Age: < 1 day
- Feeding: Desmodesmus subspicatus
- Feeding during test: none
- Control group: negative control (water only), positive control: potassium dichromate
STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Vehicle, solvent: water
- Concentration of vehicle/ solvent: 1000 mg/l
STABILITY OF THE TEST CHEMICAL SOLUTIONS: see stability information (hydrolysis as function of pH).
DILUTION WATER
- Source: Synthetic fresh water
- Aeration: no
- Hardness: CaCl2 x 2 H2O: 294 mg/l, MgSO4 x 7 H2O: 123 mg/l
- Salinity: KCl: 5.5 mg/l
- Ca/Mg ratio: 4 : 1
- Na/K ratio: 10 : 1
- pH: 7.0 to 7.5
- Oxygen content: 6.6 to 7.8 mg/l
TEST SYSTEM
- Test type: static
- Concentrations: (nominal): 100, 140, 200, 280, 400, 560, 800 mg/l
- Exposure vessel type: round bottom flasks
- Number of replicates, individuals per replicate: 4 replicates, 5 individuals
- Test temperature: 20 +- 2°C
- Dissolved oxygen: 6.6 - 7.8 mg/l
- pH: 7.0 - 7.5
- Adjustment of pH: no
- Intensity of irradiation: dark
DURATION OF THE TEST: 48 hours
TEST PARAMETER: Immobilization
MONITORING OF TEST SUBSTANCE CONCENTRATION: not performed, nominal concentrations used.
Statistical Analyis: Probit analysis according to Cavalli-Sforza

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

202.3 mg/L

Nominal / measured:

nominal

Remarks on result:

other: 175.2-233.6 mg/L

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

400 mg/L

Nominal / measured:

nominal

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

285.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: 244.6-333.9 mg/L

Details on results:

Based on effective concentrations (corrected for hydrolysis) the EC50 48 h is 179 mg/l.

Validity criteria fulfilled:

not specified

Conclusions:

Based on effective concentrations (corrected for hydrolysis) the EC50 48 h is 179 mg/l.

Description of key information

Based on effective concentrations (corrected for hydrolysis) the EC50 48 h is 179 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

179 mg/L