Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

72 h ErC10: 7.8 mg/L
72 h ErC50: >93 mg/L

Key value for chemical safety assessment

EC50 for freshwater algae:
93 mg/L
EC10 or NOEC for freshwater algae:
7.8 mg/L

Additional information

In the Klimisch 1 GLP study from Eckenstein (2013) the toxicity of Butanedioic acid, sulfo-, 1,4-diisodecyl ester, sodium salt to Desmodesmus subspicatus was determined in a static 72-Hour Algal Growth Inhibition Test. The test was performed according to OECD 201 and EU Method C.3. The nominal concentrations of the test item of 0.52, 1.6, 5.2, 16, 52 and 164 mg/L (corresponding to 0.32, 1.0, 3.2, 10, 32 and 100 mg sulfosuccinate/L) were tested in parallel with a control. Dose verification analysis was performed . The measured concentrations of the test item in the test media of the nominal test concentrations of 5.2 to 164 mg/L were between 78 and 104 % of the nominal values at the start of the test. During the test period of 72 hours, a decrease of test item concentration in the test media occurred. At the end of the test, <LOQ (LOQ=2.89 mg/L) to 86% of the nominal values were found. Losses of the test item may result due to adsorption of the test item onto the algae and the test vessels, particularly in the low-level samples. In order to determine the growth of the cultures, the cells were counted using a coulter counter. The cell density was determined at 0, 24, 48 and 72 hours. The growth of the control cultures fulfilled the validity criteria from OECD 201 (2006). The 72 -hour ErC10 and ErC50 are 7.8 and > 93 mg active substance/L (highest tested concentration) based on the mean measured concentration.

This information is considered to be relevant and reliable for the further risk assessment.