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EC number: 939-538-4 | CAS number: -
Observations in the Test Vessels
Nominal test itemconcentration[mg/L]
Number of fish effected at observation time [hours]
Measured Concentrations of Sodium methyl oleyl taurate (C18:1-part) in Aqueous Phase and at Glass Walls of Test Solutions at 0 and 24 hours
(Volume: 10 L)
of 24 hours
Meas. conc. a.i.
Measured Concentrations of Sodium methyl oleyl taurate (C18:1-part) in Aqueous Phase and at Glass Walls of Test Solutions at 72 and 96 hours
The acute toxicity of the test item Sodium methyl oleyl taurate(batch no.: DEB4003485)to fish (zebrafish) was determined according to OECD-Guideline for Testing of Chemicals No. 203 (1992) from 2012-12-10 to 2013-02-08 with the definitive exposure phase from 2013-02-04 to 2013-02-08 at Dr.U.Noack-Laboratorien, 31157 Sarstedt, Germany.
A semi-static test with daily renewal of the test media was performed with nominal test item concentrations of 0.156 - 0.313 - 0.625 - 1.25 - 2.5 mg/L. Duration of the test was 96 hours. 7 test organisms were exposed to each concentration and the control. Water quality parameters pH-value, temperature and oxygen-saturation, measured after 0, 24, 48, 72 and 96 h, were determined to be within the acceptable limits.
The concentrations of the active ingredient of Sodium methyl oleyl taurate(C18:1-part) were analytically verified by LC-MS/MS at the start of the exposure (0 h and 72 h) and at the end of the exposure (24 h and 96 h) of all concentration levels and the control. The measured concentrations of active ingredient at the start of the exposure were in the range of 91 to 119 % of the nominal values. The measured concentrations of the active ingredient at the end of the exposure were in the range of 61 to 119 % of the nominal values, indicating that the concentrations of the active ingredient were successfully maintained for the duration of the test. The endpoints were based on the nominal concentrations of the test item Sodium methyl oleyl taurate and additionally given on the nominal active ingredient (Table 1 and Table 2).
Adsorption of the test item to the test vessels were analytically measured (refer to 4.2.4). The measured amount of adsorbed test item was below 10 % in all cases.
IMPORTANT NOTE TO AVOID CONFUSION:
As the registration substance is a UVCB this means that the test item is 100% active ingredient.
All effect levels are given based on the nominal concentrations of the test item
LC-Values (0 – 96 hours)
Based on nominal test item concentrations [mg/L]
2.32 (1.25* - 2.50**)
2.37 (1.25* - 2.50**)
2.45 (1.25* - 2.50**)
1.25* LC10 2.50***
1.25* LC20 2.50***
1.77 (1.25* - 2.50***)
1.24 (1.24 – 1.24)
1.27 (1.27 – 1.27)
1.32 (1.31 – 1.32)
Highest test item concentration with0 % mortality after96 hours
Lowest test item concentration with100 % mortality after96 hours
*Concentration with 0 % mortality
**Concentration with 71 % mortality
***Concentration with 100 % mortality
All effect levels are given based on the nominal concentrations of the test item Sodium methyl oleyl taurate and its active ingredient. The test item was found to be toxic to zebrafish with a LC50 after 96 hours of 1.32 (1.31 – 1.32) mg test item/L, corresponding to 0.810 (0.800 – 0.810) mg a.i./L. At the nominal test item concentration of 0.625 mg/L (0.381 mg a.i./L) no mortality or non lethal effects were observed.
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