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EC number: 205-553-3 | CAS number: 142-71-2
- Life Cycle description
- Uses advised against
- Endpoint summary
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- Endpoint summary
- Stability
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
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- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
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- Toxicological Summary
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- Irritation / corrosion
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- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with Good laboratory Practice and internationally accepted guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Copper di(acetate)
- EC Number:
- 205-553-3
- EC Name:
- Copper di(acetate)
- Cas Number:
- 142-71-2
- Molecular formula:
- Cu(CO2CH3)2
- IUPAC Name:
- copper di(acetate)
- Details on test material:
- - Name of test material (as cited in study report): Copper acetate monohydrate.
- Analytical purity: 99.9%.
- Lot/batch No.: 11.6.1SD
- Storage condition of test material: room temperature in the dark.
Constituent 1
Results and discussion
In vitro
Resultsopen allclose all
- Irritation / corrosion parameter:
- other: other: Relative mean viability
- Remarks on result:
- other: Basis: other: reconstructed human epidermis model. Time point: 240 minute exposure period. Remarks: The relative mean viability of the test item treated tissues was 12.1% after a 240 minute exposure period.
- Irritation / corrosion parameter:
- other: other: Relative mean viability
- Remarks on result:
- other: Basis: other: reconstructed human epidermis model. Time point: 60 minute exposure period. Remarks: The relative mean viability of the test item treated tissues was 21.4% after a 60 minute exposure period.
- Irritation / corrosion parameter:
- other: other: Relative mean viability
- Remarks on result:
- other: Basis: other: reconstructed human epidermis model. Time point: 3 minute exposure period. Remarks: The relative mean viability of the test item treated tissues was 54.4% after a 3 minute exposure period.
Any other information on results incl. tables
RESULTS
Direct MTT Reduction
The MTT solution containing the test item did not turn blue which indicated that the test item did not directly reduce MTT. Quantitative correction of the results was therefore unnecessary.
Test Item, Positive Control Item and Negative Control Item
Mean OD540 values and viabilities for the negative control, positive control and test item are given in Table 1 (attached).
The relative mean viability of the test item treated tissues was as follows:
240 minutes exposure: 12.1%
60 minutes exposure: 21.4%
3 minutes exposure: 54.4%
The 240 and 60 minute exposures induced tissue viabilities less than 35%. Therefore according to the prediction model a corrosive classification was triggered. The 3 minute exposure was greater than 35% and therefore the test item did not trigger a corrosive classification after a 3 minute exposure.
Quality Criteria
The relative mean tissue viability for the positive control treated tissues was 7.3% relative to the negative control treated tissues following the 240-Minute exposure period. The positive control acceptance criterion was therefore satisfied.
The mean OD540 for the negative control treated tissues was 0.206. The negative control acceptance criterion was therefore satisfied.
The test item was classified as corrosive to the skin.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1B (corrosive)
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Classification according to Directive 67/548/EEC: Corrosive (C). R34, Causes burns.
Classification according to CLP/GHS: Category 1B Corrosive, H314, Causes severe skin burns and eye damage. - Executive summary:
A GLP-compliant study was conducted in accordance with OECD Guideline 431 and EU Method B.40 BIS. Duplicate tissues were treated with the test item for exposure periods of 3, 60 and 240 minutes. At the end of the exposure period the test item was rinsed from each tissue before each tissue was taken for MTT-Ioading. After MTT loading a total biopsy of each epidermis was made and placed into micro tubes containing acidified isopropanol for extraction of formazan crystals out of the MTT -loaded tissues. At the end of the formazan extraction period each tube was mixed thoroughly and duplicate 200 µl samples were transferred to the appropriate wells of a pre-Iabelled 96-well plate. The optical density (OD) was measured at 540 nm (OD540). Data were presented in the form of percentage viability (MTT reduction in the test item treated tissues relative to negative control tissues). The relative mean viability of the test item treated tissues was as follows:
240 minutes exposure: 12.1%
60 minutes exposure: 21.4%
3 minutes exposure: 54.4%The quality criteria required for acceptance of results in the test were satisfied. The test item was classified as corrosive to the skin. The following classification criteria apply:
EU DSD (67/548/EEC) Corrosive requires symbol "C" risk phrase R34 "CAUSES BURNS".
EU CLP (1272/2008/EC) and UN GHS Hazard statement H314 "Causes severe skin burns and eye damage" Category 1 B.
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