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Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: reference study data from SNIF file

Data source

Referenceopen allclose all

Reference Type:
other: SNIF data provided by ECHA
Title:
Unnamed
Report date:
1990
Reference Type:
secondary source
Title:
Unnamed
Year:
1990

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
GLP compliance:
yes
Type of assay:
micronucleus assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(phenylmethoxy)naphthalene
EC Number:
405-490-3
EC Name:
2-(phenylmethoxy)naphthalene
Cas Number:
613-62-7
Molecular formula:
C17 H14 O
IUPAC Name:
2-(benzyloxy)naphthalene

Test animals

Species:
mouse
Strain:
NMRI
Sex:
male/female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
corn oil
Duration of treatment / exposure:
animals given 200, 670 or 2000 mg/kg bw ( 5 males and 5 females in each group): 24 h
5 males and 5 females given 2000 mg/kg bw: 48 h
Frequency of treatment:
once
Post exposure period:
24/ 48 h
Doses / concentrations
Remarks:
Doses / Concentrations:
0, 200, 670 or 2000 mg/kg bw
Basis:
no data
No. of animals per sex per dose:
5 (200, 670 mg/kg bw)
10 (2000 mg/kg bw)
Control animals:
yes, concurrent vehicle
Positive control(s):
30 mg/kg of cyclophosphamide

Examinations

Tissues and cell types examined:
Bone marrow smears were made from each animal and 1000 polychromatic erythrocytes were scored for micronuclei.

Results and discussion

Test results
Sex:
male/female
Genotoxicity:
negative
Toxicity:
no effects
Remarks:
however in a preliminary toxicity study, signs of toxicity occurring at 2000 mg/kg/d included reduction of spontaneous activity, eyelid closure and apathy for up to 6 hours following treatment
Vehicle controls validity:
valid
Negative controls validity:
not specified
Positive controls validity:
valid
Additional information on results:
No cytotoxicity was reported in any treatment or control assays. No significant increase in the number of micronucleated polychromatic erythrocytes was reported in treatment assays. The positive control induced micronuclei as expected.

Applicant's summary and conclusion

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